Abstract

In this study, classical and molecular methods were involved for isolation and identification of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA). Identification of S.aureus involved tests such as Mannitol fermentation test, coagulase test and DNase test. Antibiotic susceptibility test which was carried out to explore MRSA antibiotic susceptibility patterns. Antibiotics involved were methicillin, oxacillin, vancomycin, tetracycline, cefoxitin, erythromycin, gentamycin, chloramphenicol, penicillin, ampicillin, and trimethoprim. Polymerase chain reaction (PCR) was performed to obtain more reliable result. Among 100 samples collected, totally 18 samples were confirmed as S. aureus based on molecular method. From these isolates, 16 of them showed presence of mecA gene and also fem gene(gene involved in the resistant of methicillin) thus making it positive as MRSA.