(2017) Assessment of biofilm formation in Pseudomonas aeruginosa by antisense mazE-PNA. Microbial Pathogenesis. pp. 28-31. ISSN 0882-4010
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Abstract
The hallmark patogenicity in Pseudomonas aeruginosa (P. aeruginosa) is biofilm formation that is not easy to eradicate, because it has variety mechanisms for antibiotic resistance. In addition, toxin-antitoxin (TA) system may play role in biofilm formation. The current study aimed to evaluate the role of TA loci in biofilm formation. Therefore, 18 P. aeruginosa clinical isolates were collected and evaluated for specific biofilm and TA genes. The analysis by RT-qPCR demonstrated that expression of mazE antitoxin in biofilm formation was increase. On the other hand, mazE antitoxin TA system was used as target for antisense PNA. mazE-PNA was able to influence in biofilm formation and was inhibit at 5,10 and 15 mu M concentrations biofilm formation in P aeruginosa. Therefore, it could be highlighted target for anti-biofilm target to eradicate P. aeruginosa biofilm producer. (C) 2017 Elsevier Ltd. All rights reserved.
| Item Type: | Article | ||||||||
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| Keywords: | Biofilm TAsystem PNA P. aeruginosa Immunology Microbiology | ||||||||
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| Page Range: | pp. 28-31 | ||||||||
| Journal or Publication Title: | Microbial Pathogenesis | ||||||||
| Journal Index: | ISI | ||||||||
| Volume: | 104 | ||||||||
| Identification Number: | https://doi.org/10.1016/j.micpath.2017.01.009 | ||||||||
| ISSN: | 0882-4010 | ||||||||
| Depositing User: | مهندس مهدی شریفی | ||||||||
| URI: | http://eprints.medilam.ac.ir/id/eprint/344 |
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