Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Prevalence of carbapenemases and ESBL encoding genes among K. pneumoniae isolates obtained from an educational hospital in Ahvaz, Southwestern Iran

Fri Mar 29 17:01:34 2024

(2021) Prevalence of carbapenemases and ESBL encoding genes among K. pneumoniae isolates obtained from an educational hospital in Ahvaz, Southwestern Iran. Gene Reports. p. 5.

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Background: K. pneumoniae is a gram-negative pathogen that common cause of nosocomial infections. The rapid spread of multi-drug resistant (MDR) K. pneumoniae is an important issue. This study aimed to identify carbapenemases (Klebsiella pneumoniae carbapenemase KPC, oxacillinase- 48 OXA-48, New Delhi metallo-8-lactamase-1 NDM-1), oxacillinase-181 OXA-181 and extended-spectrum beta-lactamases (ESBL) (CTX-M-15) were identified among K. pneumoniae isolates in Ahvaz, Iran. Methods: In this cross-sectional study, conducted from July 2018 to July 2019, isolates of K. pneumoniae obtained from various clinical samples. All Gram-negative bacilli isolates were differentiated on species level by standard biochemical tests. In following, antibiotic susceptibility testing by disk diffusion method, ESBLs, carbapenemase, metallo-beta-lactamase and bla(CTX-M-15), bla(KPC), bla(OXA-48), bla(NDM), and bla(OXA-181) genes were performed. Results: In total, 120 clinical isolates of K. pneumoniae were obtained. The most effective antibiotics for K. pneumoniae isolates were colistin, imipenem, and doripenem, with 98.3%, 64.1%, and 64.1% susceptibility. Thirty-one isolates (25%) were resistant to meropenem disc and considered as carbapenem-resistant. A total of 78 isolates (65%) were ESBLs by double-disc synergy test (DDST) and 25 isolates (20.8%) produced metallo-betalactamase. Among 31 (25%) carbapenem-resistant isolates, 22 isolates (71%) hydrolyzed the meropenem and classified as carbapenemase producers. PCR assays revealed that prevalence of bla(CTX-M-15), bla(KPC), bla(OXA-48), bla(NDM), and bla(OXA-181) genes was 36.7%, 10%, 64.15%, 1.7% and 8.3%, respectively. Conclusion: This study found a high prevalence of MDR K. pneumoniae harboring MBL and ESBL genes. It is recommended that local periodic. Surveys are performed for controlling antimicrobial resistance.

Item Type: Article
Creators:
CreatorsEmail
Sheikh, A. F.UNSPECIFIED
Khoshnood, S.UNSPECIFIED
Saki, M.UNSPECIFIED
Shahi, F.UNSPECIFIED
Seyed-Mohammadi, S.UNSPECIFIED
Abdi, M.UNSPECIFIED
Moradi, M.UNSPECIFIED
Keywords: blaNDM ESBL K. pneumoniae MBL spectrum beta-lactamase intensive-care units klebsiella-pneumoniae escherichia-coli epidemiology emergence dissemination bla(oxa-181) resistance pattern Genetics & Heredity
Divisions:
Page Range: p. 5
Journal or Publication Title: Gene Reports
Journal Index: ISI
Volume: 23
Identification Number: https://doi.org/10.1016/j.genrep.2021.101128
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/3433

Actions (login required)

View Item View Item