(2020) Inhibitory effects of Lactobacilli cocktail on HT-29 colon carcinoma cells growth and modulation of the Notch and Wnt/β-catenin signaling pathways. Microbial Pathogenesis. ISSN 08824010 (ISSN)
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Abstract
Probiotics could be considered as attractive candidates for preventing tumor growth through maintaining homeostasis. The aim of this study was to evaluate the inhibitory effect of a cocktail of five Lactobacillus species on human colorectal carcinoma cell line HT-29. The anti-proliferative and apoptotic effects of Lactobacilli cocktail were evaluated using MTT and flow cytometry tests, respectively. Quantitative real-time polymerase chain reaction (qPCR) was used to analyze the expression of several genes in the Notch (notch, hes1, msi1, and numb) and Wnt/β-catenin (CTNNB1 and CCND1) pathways, following the treatment of HT-29 cells with Lactobacilli cocktail. The treatment by Lactobacilli cocktail induced a significant anti-proliferative effect and late stage apoptosis among the cancer cells (p < 0.05). Compared to the untreated cells, Lactobacilli cocktail induced the down-regulation of notch, hes1, and msi1 genes and up-regulation of numb gene in the Notch pathway as well as the down-regulation of CTNNB1 and CCND1 genes in the Wnt/β-catenin pathway in a time-dependent manner (p < 0.05). Conclusion: Lactobacilli cocktail was shown to have beneficial anti-tumor effects on HT-29 cells by modulating the Notch and Wnt/β-catenin pathways; therefore, the use of Lactobacilli probiotics as nutritional supplements may prevent the incidence of colon cancer. © 2019
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| Keywords: | Anti-proliferation Apoptosis Lactobacilli Notch Wnt/β-catenin beta catenin cyclin D1 Notch receptor transcription factor HES 1 Wnt protein antineoplastic activity antiproliferative activity Article cancer inhibition CCND1 gene cell viability colon carcinoma controlled study CTNNB1 gene diet supplementation down regulation flow cytometry gene gene expression hes1 gene HT-29 cell line human human cell IC50 Lactobacillus Lactobacillus brevis Lactobacillus plantarum Lactobacillus reuteri Lactobacillus rhamnosus msi1 gene MTT assay nonhuman notch gene numb gene priority journal real time polymerase chain reaction signal transduction upregulation | ||||||||||||||
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| Journal or Publication Title: | Microbial Pathogenesis | ||||||||||||||
| Journal Index: | Scopus | ||||||||||||||
| Volume: | 139 | ||||||||||||||
| Identification Number: | https://doi.org/10.1016/j.micpath.2019.103829 | ||||||||||||||
| ISSN: | 08824010 (ISSN) | ||||||||||||||
| Depositing User: | مهندس مهدی شریفی | ||||||||||||||
| URI: | http://eprints.medilam.ac.ir/id/eprint/2857 |
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