Abstract

The extract of Allium saralicum R.M. Fritsch leaf was found to be an effective reagent for green synthesis and functionalization of zinc nanoparticles (ZnNPs@AS) from 1 mM zinc acetate solution. These nanoparticles were characterized by fourier-transform infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), field emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM) analysis. FT-IR results offered polysaccharides and protein in A. saralicum were the sources of reducing power, reducing zinc ions to ZnNPs@AS. In XRD analysis, the crystal size of zinc nanoparticles was 19.7 nm. TEM and FE-SEM images indicated the average diameter of 19 nm for the zinc nanoparticles. For investigating the antimicrobial properties of Zn (NO3)(2).6H(2)O, A. saralicum, and ZnNPs@AS, we used the macro-broth dilation test. Also, MIC, MBC, and MFC of Zn (NO3)(2).6H(2)O, A. saralicum, and ZnNPs@AS were gained to indicate the exact antimicrobial potentials of them. ZnNPs@AS indicated higher antifungal and antibacterial effects than all standard antibiotics (p <= 0.01). The MIC of ZnNPs@AS were 2-4 and 2-8 mg/mL against fungi and bacteria, respectively. But, MBC and MFC of ZnNPs@AS were 4-8 mg/mL against all fungi and bacteria tested. To survey the cutaneous wound healing effects of Zn (NO3)(2).6H(2)O, A. saralicum, and ZnNPs@AS, the 0.2 ointments were formulated and compared with the control and untreated groups. The use of ZnNPs@AS ointment significantly (p <= 0.01) raised the wound contracture, vessel, hydroxyl proline, hexuronic acid, hexosamine, fibrocyte, fibroblast, and fibrocytes/fibroblast rate and significantly (p <= 0.01) decreased the wound area, total cells, neutrophil, macrophage, and lymphocyte compared to other groups in rats. According to the above findings, ZnNPs@AS may be consumed for the treatment of cutaneous wounds and infectious diseases in humans.