(2007) Dissemination of a single Vibrio cholerae clone in cholera outbreaks during 2005 in Iran. Journal of Medical Microbiology. pp. 1615-1619. ISSN 00222615 (ISSN)
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Abstract
In this study, 50 Vibrio cholerae O1 serotype Inaba isolates were collected during several cholera outbreaks throughout Iran during the summer of 2005. The results of antibiotic susceptibility testing showed that 86, 84, 84 and 82 of the isolates were resistant to streptomycin, chloramphenicol, co-trimoxazole and tetracycline, respectively. The strains were genotyped using randomly amplified polymorphic DNA (RAPD), PFGE and ribotyping techniques. PCR showed that 100, 98 and 98 carried the ctx, zot and ace genes, respectively. Biochemical fingerprinting of the isolates using the PhenePlate (PhP) system showed a low diversity index level (0.755), suggesting that the strains were highly homogeneous. Among the strains, 100 and 96 showed an identical ribotype and PFGE patterns, respectively. The two isolates showing different PFGE patterns also exhibited discrete PhP types. RAPD was able to discriminate the isolates into six distinct groups, suggesting some genetic dissimilarity was present among the strains. These ribotyping, PFGE and PhP techniques revealed the clonal dissemination of a single V. cholerae strain throughout Iran in 2005. © 2007 SGM.
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| Keywords: | ampicillin chloramphenicol ciprofloxacin cotrimoxazole doxycycline erythromycin furazolidone gentamicin polymyxin B streptomycin tetracycline antibiotic sensitivity article bacterium isolate cholera controlled study DNA fingerprinting epidemic genetic difference genetic variability genotype human Iran nonhuman plate count polymerase chain reaction priority journal pulsed field gel electrophoresis random amplified polymorphic DNA ribotyping Vibrio cholerae Bacterial Typing Techniques Disease Outbreaks DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Epidemiology, Molecular Microbial Sensitivity Tests Random Amplified Polymorphic DNA Technique | ||||||||||||||||||
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| Page Range: | pp. 1615-1619 | ||||||||||||||||||
| Journal or Publication Title: | Journal of Medical Microbiology | ||||||||||||||||||
| Journal Index: | Scopus | ||||||||||||||||||
| Volume: | 56 | ||||||||||||||||||
| Number: | 12 | ||||||||||||||||||
| Identification Number: | https://doi.org/10.1099/jmm.0.47218-0 | ||||||||||||||||||
| ISSN: | 00222615 (ISSN) | ||||||||||||||||||
| Depositing User: | مهندس مهدی شریفی | ||||||||||||||||||
| URI: | http://eprints.medilam.ac.ir/id/eprint/1668 |
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