(2011) Recombinant streptodornase production in Escherichia. coli. Australian Journal of Basic and Applied Sciences. pp. 2343-2347. ISSN 19918178 (ISSN)
Full text not available from this repository.
Abstract
Introduction: In pyoderma infections, pus of density related to desoxiribonucleoproteins. streptodornase(sd) combination with streptokinase dissolve purulent secretions from infections.This study aims to produce recombinant streptodornase of Streptococcus pyogenes in E.coli. Methods: by using specific primers streptodornase gene (sd) was amplified. The constructed vector pGEX4T1-sd was transformed in E. coli BL21 (DE3)/ pLYS-S and induced with IPTG. Recombinant protein was purified by affinity-chromatography (Glutathione Sepharose 4B). Results: a PCR product about 816 bp was seen. Sd recombinant protein was expressed in pGEX-4T-1 in E. coli BL21. SDS-PAGE analysis of product before and after inducing indicated a band about 60 kDs. A High concentration of the recombinant protein obtained from the single-step purification by affinity-chromatography (Glutathione Sepharose 4B), and purified recombinant Sd protein appeared at about 38.2 kDa. Conclusion: results showed Sd recombinant protein has antigenic characteristics and it can be used for diagnostic aims.
| Item Type: | Article | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Creators: |
|
||||||||||
| Keywords: | Recombinant Streptococcus Streptodornase | ||||||||||
| Divisions: | |||||||||||
| Page Range: | pp. 2343-2347 | ||||||||||
| Journal or Publication Title: | Australian Journal of Basic and Applied Sciences | ||||||||||
| Journal Index: | Scopus | ||||||||||
| Volume: | 5 | ||||||||||
| Number: | 12 | ||||||||||
| ISSN: | 19918178 (ISSN) | ||||||||||
| Depositing User: | مهندس مهدی شریفی | ||||||||||
| URI: | http://eprints.medilam.ac.ir/id/eprint/1617 |
Actions (login required)
 |
View Item |