Abstract

The periodontium is a supportive structure which surrounds the teeth and supports them. This structure consists of different tissues including gingiva, cementum, periodontal ligament and alveolar bone. The aim of this study is to analyze the osteogenic stimulatory effect of the combination of dexamethasone and L.L.L.I. on the periodontal stem cell (PDLSc). The PDLSc were obtained from the first premolar root of young healthy patients who had their teeth extracted for orthodontic treatment purposes. After obtaining the stem cells, the PDLSc were cultured in plates containing α-MEM and 15 FBS. The osteogenic differentiation was carried out in three groups, basic medium, Dexamethasone and L.L.L.I. and negative control and then the alizarin red staining test, calcium test and alkaline phosphatase test were performed. The results showed that intracellular calcium and alkaline phosphatase in the dexamethasone and L.L.L.I. group was significantly higher than the control group (p<0.05). Also the stain test showed mineralization of cells in the dexamethasone and L.L.L.I. group. The results of this study showed that the combination of dexamethasone and L.L.L.I. has a stimulatory osteogenic effect on PDLSc.