(2015) Acinetobacter baumannii clonal lineages I and II harboring different carbapenem-hydrolyzing-β-lactamase genes are widespread among hospitalized burn patients in Tehran. Journal of Infection and Public Health. pp. 533-542. ISSN 18760341 (ISSN)
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Abstract
The aim of this study was to analyze antimicrobial resistance patterns and their encoding genes and genotypic diversity of Acinetobacter baumannii isolated from burn patients in Tehran, Iran. The presence of extended-spectrum beta-lactamase- and blaOXA-encoding genes among 37 multidrug resistant (MDR) A. baumannii strains isolated from patients hospitalized in a teaching hospital in Tehran was evaluated. Susceptibility to 7 antibiotics was tested by disk agar diffusion and to polymyxin B and colistin was tested by E-test, according to CLSI guidelines. All isolates were then analyzed by PCR for the presence of blaIMP, blaVIM, blaSIM blaOXA-23, blaOXA-24, and blaOXA-58-like carbapenemase genes, and blaOXA-51-like, blaTEM, blaSHV, blaPER, blaVEB, and blaGIM genes. Genotyping of A. baumannii strains was performed by repetitive sequence-based (REP)-PCR and cluster analysis of REP-PCR profiles. A. baumannii isolates were assigned to international clones by multiplex PCR sequence group analysis. Twenty-five A. baumannii isolates were classified as MDR, and 12 were classified as extensively drug resistant. All isolates were susceptible to colistin and polymyxin B. Eighty-one percent of the isolates was resistant to imipenem or meropenem and harbored at least one or both of the blaOXA-23-like or blaOXA-24-like carbapenemase genes. Co-existence of different resistance genes was found among carbapenem-resistant isolates. Multiplex PCR sequence group analysis most commonly assigned A. baumannii isolates to international clones I (18/37; 48.6) and II (18/37; 48.6). An alarming increase in resistance to carbapenems and the spread of blaOXA-23-like and/or blaOXA-24-like carbapenemase genes was observed among A. baumannii strains belonging to clonal lineages I and II, isolated from burn patients in Tehran. © 2015 King Saud Bin Abdulaziz University for Health Sciences.
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| Keywords: | Acinetobacter baumannii Burn Lineage OXA gene carbapenem colistin extended spectrum beta lactamase imipenem meropenem polymyxin B antiinfective agent bacterial protein beta lactamase carbapenemase antibiotic resistance antibiotic sensitivity Article burn patient clone disk diffusion epsilometer test female genetic variability genotype hospital patient human Iran male nonhuman polymerase chain reaction priority journal Acinetobacter Infections classification cluster analysis complication enzymology genetics isolation and purification microbiology multidrug resistance multilocus sequence typing retrospective study teaching hospital Anti-Bacterial Agents Bacterial Proteins beta-Lactamases Burns Disk Diffusion Antimicrobial Tests Drug Resistance, Multiple, Bacterial Hospitals, Teaching Humans Retrospective Studies | ||||||||||||||||||||
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| Page Range: | pp. 533-542 | ||||||||||||||||||||
| Journal or Publication Title: | Journal of Infection and Public Health | ||||||||||||||||||||
| Journal Index: | Scopus | ||||||||||||||||||||
| Volume: | 8 | ||||||||||||||||||||
| Number: | 6 | ||||||||||||||||||||
| Identification Number: | https://doi.org/10.1016/j.jiph.2015.04.030 | ||||||||||||||||||||
| ISSN: | 18760341 (ISSN) | ||||||||||||||||||||
| Depositing User: | مهندس مهدی شریفی | ||||||||||||||||||||
| URI: | http://eprints.medilam.ac.ir/id/eprint/1439 |
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