Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Isolation and Identification of Methicillin-resistance Staphylococcus aureus (MRSA) from Nasal of College Students

Wed Nov 30 17:12:39 2022

(2013) Isolation and Identification of Methicillin-resistance Staphylococcus aureus (MRSA) from Nasal of College Students. Journal of Pure and Applied Microbiology. pp. 3155-3162. ISSN 0973-7510

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Abstract

In this study, classical and molecular methods were involved for isolation and identification of Staphylococcus aureus (S. aureus) and methicillin-resistant S. aureus (MRSA). Identification of S.aureus involved tests such as Mannitol fermentation test, coagulase test and DNase test. Antibiotic susceptibility test which was carried out to explore MRSA antibiotic susceptibility patterns. Antibiotics involved were methicillin, oxacillin, vancomycin, tetracycline, cefoxitin, erythromycin, gentamycin, chloramphenicol, penicillin, ampicillin, and trimethoprim. Polymerase chain reaction (PCR) was performed to obtain more reliable result. Among 100 samples collected, totally 18 samples were confirmed as S. aureus based on molecular method. From these isolates, 16 of them showed presence of mecA gene and also fem gene(gene involved in the resistant of methicillin) thus making it positive as MRSA.

Item Type: Article
Creators:
CreatorsEmail
Amini, R.UNSPECIFIED
Abdulamir, A. S.UNSPECIFIED
Mustaffa, N. A. B.UNSPECIFIED
Jahanshiri, F.UNSPECIFIED
Ling, B. P.UNSPECIFIED
Hematian, A.UNSPECIFIED
Taheri-Kalani, M.UNSPECIFIED
Amini, Y.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Sekawi, Z.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Keywords: MRSA nasal Staphylococcus antibiotics multiplex pcr genes malaysia agar meca Biotechnology & Applied Microbiology Microbiology
Divisions:
Page Range: pp. 3155-3162
Journal or Publication Title: Journal of Pure and Applied Microbiology
Journal Index: ISI
Volume: 7
Number: 4
ISSN: 0973-7510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/692

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