Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Recombinant streptodornase production in Escherichia. coli

Tue Jul 5 16:25:48 2022

(2011) Recombinant streptodornase production in Escherichia. coli. Australian Journal of Basic and Applied Sciences. pp. 2343-2347. ISSN 19918178 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Introduction: In pyoderma infections, pus of density related to desoxiribonucleoproteins. streptodornase(sd) combination with streptokinase dissolve purulent secretions from infections.This study aims to produce recombinant streptodornase of Streptococcus pyogenes in E.coli. Methods: by using specific primers streptodornase gene (sd) was amplified. The constructed vector pGEX4T1-sd was transformed in E. coli BL21 (DE3)/ pLYS-S and induced with IPTG. Recombinant protein was purified by affinity-chromatography (Glutathione Sepharose 4B). Results: a PCR product about 816 bp was seen. Sd recombinant protein was expressed in pGEX-4T-1 in E. coli BL21. SDS-PAGE analysis of product before and after inducing indicated a band about 60 kDs. A High concentration of the recombinant protein obtained from the single-step purification by affinity-chromatography (Glutathione Sepharose 4B), and purified recombinant Sd protein appeared at about 38.2 kDa. Conclusion: results showed Sd recombinant protein has antigenic characteristics and it can be used for diagnostic aims.

Item Type: Article
Creators:
CreatorsEmail
Kamani, M.UNSPECIFIED
Abtahi, H.UNSPECIFIED
Nazari, R.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Keywords: Recombinant Streptococcus Streptodornase
Divisions:
Page Range: pp. 2343-2347
Journal or Publication Title: Australian Journal of Basic and Applied Sciences
Journal Index: Scopus
Volume: 5
Number: 12
ISSN: 19918178 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1617

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