Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Analyzing the osteogenic stimulatory effect of the combination dexamethasone and low levelled laser irradiation(L.L.L.I) on periodontal ligament stem cell(PDLSc)

Sat Jun 25 03:05:14 2022

(2015) Analyzing the osteogenic stimulatory effect of the combination dexamethasone and low levelled laser irradiation(L.L.L.I) on periodontal ligament stem cell(PDLSc). Der Pharma Chemica. pp. 226-230. ISSN 0975413X (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

The periodontium is a supportive structure which surrounds the teeth and supports them. This structure consists of different tissues including gingiva, cementum, periodontal ligament and alveolar bone. The aim of this study is to analyze the osteogenic stimulatory effect of the combination of dexamethasone and L.L.L.I. on the periodontal stem cell (PDLSc). The PDLSc were obtained from the first premolar root of young healthy patients who had their teeth extracted for orthodontic treatment purposes. After obtaining the stem cells, the PDLSc were cultured in plates containing α-MEM and 15 FBS. The osteogenic differentiation was carried out in three groups, basic medium, Dexamethasone and L.L.L.I. and negative control and then the alizarin red staining test, calcium test and alkaline phosphatase test were performed. The results showed that intracellular calcium and alkaline phosphatase in the dexamethasone and L.L.L.I. group was significantly higher than the control group (p<0.05). Also the stain test showed mineralization of cells in the dexamethasone and L.L.L.I. group. The results of this study showed that the combination of dexamethasone and L.L.L.I. has a stimulatory osteogenic effect on PDLSc.

Item Type: Article
Creators:
CreatorsEmail
Ali Roozegar, M.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Mohammadi, T. M.UNSPECIFIED
Hoshmand, B.UNSPECIFIED
Keywords: Alizarin red Alkaline phosphatase Dexamethasone L.L.L.I. PDLSC calcium Article calcium cell level cell differentiation cell stimulation controlled study human human cell in vitro study low level laser therapy mineralization normal human periodontium stem cell culture
Divisions:
Page Range: pp. 226-230
Journal or Publication Title: Der Pharma Chemica
Journal Index: Scopus
Volume: 7
Number: 11
ISSN: 0975413X (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/1487

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