Abstract

BACKGROUND AND OBJECTIVE: Today, resistance to multiple classes of antibiotics, and notably to the beta-lactam and aminoglycosides in A. baumannii is becoming great problem and it's necessitate to make new approach to combat with multidrug-resistant (MDR), extensive drug-resistance (XDR) or Pandrug-resistant (PDR) isolates. In this case new strategy and ways should be designed and introduce against such infections. Therefore the aim of present study was evaluation of antibacterial activity of nanoconjugate gentamicin and amikacin with gold against clinical isolates of A. baumannii that collected from burn wound infection. METHODS: Eighteen A. baumannii collected from burn wound infections. For confirmation and detection of aminoglycoside resistant genes PCR carried out. Gold nanoparticles and nanoconjugates prepared according to protocol. For evaluation of the nanoconjugate, Dynamic light cattering, Transmission electron microscopy and FTIR Analysis were carried out. Then, the antibacterial activity of nanoconjugates were done by using micro broth dilution method, Result: prevalence of aminoglycoside resistant genes were aacC1, aphA6, aadA1, aadB genes 55.5, 22.2, 38.8 and 22.2 respectively. Synthesis of bare nanoconjugates resulted nanoparticle in a size 10 nm. Amikacin bound to Gnps showed excellent antibacterial activity (94.5) and just one isolate was intermediate resistance. Also gentamicin bound to GNPS have good antimicrobial effect (50) contrast to gentamicin alone. CONCLUSION: our study showed that a combination of amikacin and gentamicin with Gnps has a significant antibacterial efficiency against clinical isolates of A. baumannii. Gnps can be used as extraordinary molecular carriers for the targeting, and delivery the of antibiotic molecules to the specific infection.