Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Polymorphism of Foxp3 gene affects the frequency of regulatory T cells and disease activity in patients with rheumatoid arthritis in Iranian population

Thu Oct 6 08:50:19 2022

(2018) Polymorphism of Foxp3 gene affects the frequency of regulatory T cells and disease activity in patients with rheumatoid arthritis in Iranian population. Immunology Letters. pp. 16-22. ISSN 1879-0542 (Electronic) 0165-2478 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/30292536

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune disease that mainly affects joints and characterized by chronic joint inflammation and infiltration of various immune cells in the synovium. Forkhead box P3 (Foxp3)-expressing regulatory T cells (Tregs) play a crucial role in preventing autoimmunity and undesirable T cell responses. However, there are controversial reports regarding the defective function or frequency of these cells in various studies, which may be in part related to different polymorphisms of FoxP3 and influence of ethnicity on these differences. Therefore, the main subject of this study was to evaluate the association of Foxp3 gene polymorphism and Treg frequency in Iranian patients with RA. Accordingly, 240 RA patients diagnosed according to American college of rheumatology 2010 criteria and 240 normal subjects were recruited for this study. Genomic DNA was genotyped for -3279 C/A Foxp3 gene SNP using the PCR-RFLP. The frequency of Tregs and serum levels of interleukin (IL)-10, transforming growth factor (TGF)-beta, anti-cyclic citrullinated peptide (CCP) and rheumatoid factor (RF) were determined by flow cytometry and ELISA methods, respectively. The results showed a significant association of Foxp3 -3279 A allele with augmented risk of RA in Iranian patients compared to wild-type allele. While the frequencies of CA and AA genotypes were significantly higher in patients, RA patients with AA genotype had a significant lower frequency of Tregs compared to patients with CC and CA genotypes. Consistently, TGF-beta and IL-10 significantly diminished in patients with AA genotype compared to patients with CA and CC genotypes. Our findings indicated that the AA genotype of Foxp3 in RA patients is associated with downregulation of Tregs and susceptibility to RA in the Iranian population.

Item Type: Article
Creators:
CreatorsEmail
Hashemi, V.UNSPECIFIED
Farrokhi, A. S.UNSPECIFIED
Tanomand, A.UNSPECIFIED
Babaloo, Z.UNSPECIFIED
Hojjat-Farsangi, M.UNSPECIFIED
Anvari, E.UNSPECIFIED
Tahoori, M. T.UNSPECIFIED
Ezzeddini, R.UNSPECIFIED
Hosseini, A.UNSPECIFIED
Gharibi, T.UNSPECIFIED
Ghalamfarsa, G.UNSPECIFIED
Jadidi-Niaragh, F.UNSPECIFIED
Keywords: Foxp3 Polymorphism Regulatory T cells Rheumatoid arthritis
Divisions:
Page Range: pp. 16-22
Journal or Publication Title: Immunology Letters
Journal Index: Pubmed
Volume: 204
Identification Number: https://doi.org/10.1016/j.imlet.2018.10.001
ISSN: 1879-0542 (Electronic) 0165-2478 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/950

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