Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Molecular characterization of epidemic isolates of Vibrio cholerae O1 by arbitrarily primed PCR (AP-PCR)

Thu Dec 1 16:24:16 2022

(2008) Molecular characterization of epidemic isolates of Vibrio cholerae O1 by arbitrarily primed PCR (AP-PCR). Iranian Journal of Public Health. pp. 83-87. ISSN 0304-4556

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Abstract

Background: Epidemic and endemic cholera is a major public health problem for many countries. Aim of this study was to evaluate AP-PCR for investigation of clonal relatedness among the strains of Vibrio cholerae recovered from an outbreak occurred in different parts of Iran in 2005. Methods: The study was conducted during the cholera outbreak occurred in some of provinces in Iran in summer 2005. Bacterial isolation and identification was carried out according to the standard bacteriological methods. Arbitrarily primed PCR (AP-PCR) used to study the genetic relatedness between the V.cholerae isolates. Results: Thirty-nine isolates of V.cholerae O1 were identified. All isolates belonged to serotype Inaba. AP-PCR could differentiate the isolates into five groups. AP-PCR cluster types 1 and 2 were the most prevalent groups, accounting for 36 and 41, respectively, of V.cholerae isolates. Conclusion: The most of epidemic strains of V.cholerae O1 isolated in the year 2005 could be attributed to two predominant clusters including AP-PCR cluster types 1 and 2 accounting for more than 77 of isolates. In conclusion, a few epidemic clones were responsible for the apparently epidemic occurrence of cholera in provinces studied.

Item Type: Article
Creators:
CreatorsEmail
Ranjbar, R.UNSPECIFIED
Pourshafie, M. R.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Karami, A.UNSPECIFIED
Hamidian, M.UNSPECIFIED
Izadi, M.UNSPECIFIED
Parzadeh, M.UNSPECIFIED
Jonaidi, N.UNSPECIFIED
Keywords: Vibrio cholerae O1 AP-PCR molecular epidemiology el-tor strains malaysia non-o1 DNA parahaemolyticus outbreak 1990s iran Public, Environmental & Occupational Health
Divisions:
Page Range: pp. 83-87
Journal or Publication Title: Iranian Journal of Public Health
Journal Index: ISI
Volume: 37
Number: 2
ISSN: 0304-4556
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/913

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