Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Characterisation of genes encoding aminoglycoside-modifying enzymes among meticillin-resistant Staphylococcus aureus isolated from two hospitals in Tehran, Iran

Wed Sep 28 22:43:44 2022

(2009) Characterisation of genes encoding aminoglycoside-modifying enzymes among meticillin-resistant Staphylococcus aureus isolated from two hospitals in Tehran, Iran. International Journal of Antimicrobial Agents. pp. 264-265. ISSN 0924-8579

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Abstract

Meticillin-resistant Staphylococcus aureus (MRSA) is a major hospital pathogen and typically shows resistance to multiple antimicrobial agents. The susceptibility patterns of 109 MRSA isolates to aminoglycoside antibiotics were determined by the disk diffusion method. Genes encoding the aminoglycoside-modifying enzymes (AMEs) were targeted by polymerase chain reaction (PCR) assays. All isolates were also subjected to multiplex PCR to determine the distribution of staphylococcal cassette chromosome mec (SCCmec) types in the study population. The rates of resistance to various antibiotics were as follows: kanamycin, 97; tobramycin, 96; gentamicin, 87; amikacin, 93; and netilmicin, 80. The most prevalent AME genes were aac(6')-Ie-aph(2 '') (83) followed by aph(3')-IIIa (71). Coexistence of three AME genes was detected in 21 of isolates. The ant(4')-Ia gene was the least frequent AME gene among MRSA isolates (26). Of the 109 isolates, 106 (97) were identified as SCCmec type III or IIIA and 3 (3) as SCCmec type IV. The majority of MRSA isolates belonged to SCCmec III or IIIA and carried the aac(6')- Ie-aph(2 '') gene, which is consistent with results of susceptibility testing of these isolates against aminoglycosides. (C) 2008 Elsevier B. V. and the International Society of Chemotherapy. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Fatholahzadeh, B.UNSPECIFIED
Emaneini, M.UNSPECIFIED
Feizabadi, M. M.UNSPECIFIED
Sedaghat, H.UNSPECIFIED
Aligholi, M.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Jabalameli, F.UNSPECIFIED
Keywords: Meticillin-resistant Staphylococcus aureus MRSA Aminoglycoside-modifying enzymes Staphylococcal cassette chromosome mec SCCmec multiplex pcr identification Infectious Diseases Microbiology Pharmacology & Pharmacy
Divisions:
Page Range: pp. 264-265
Journal or Publication Title: International Journal of Antimicrobial Agents
Journal Index: ISI
Volume: 33
Number: 3
Identification Number: https://doi.org/10.1016/j.ijantimicag.2008.09.018
ISSN: 0924-8579
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/892

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