Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Phenotypic and Genotypic Evaluation of Aminoglycoside Resistance in Clinical Isolates of Staphylococci in Tehran, Iran

Wed Sep 28 23:42:05 2022

(2009) Phenotypic and Genotypic Evaluation of Aminoglycoside Resistance in Clinical Isolates of Staphylococci in Tehran, Iran. Microbial Drug Resistance. pp. 129-132. ISSN 1076-6294

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Aminoglycosides play an important role in the treatment of staphylococcal infections, despite the emerging widespread resistance among Staphylococcus. To determine the prevalence of aminoglycoside resistance and aminoglycoside modifying enzyme (AME) genes among infected patients at a teaching hospital in Tehran, Iran, we tested 585 Staphylococcus isolates, of which 322 were Staphylococcus aureus and 263 were coagulase-negative staphylococci, as determined by the disk diffusion method and multiplex PCR. The minimum inhibitory concentration of gentamicin for each isolate was determined by microbroth dilution. All methicillin-resistant staphylococci were mecA-positive by PCR. Of the 585 isolates, 27.6 were susceptible to gentamicin and kanamicin, 27.1 to tobramicin and amikacin, and 21.3 to netilmicin. The most prevalent AME genes included aac(6')-Ie-aph(2 '') (93.7) followed by aph(3')-IIIa (84.3) and ant (4')-Ia (28.1). More than 90 of aminoglycoside-resistant staphylococci contained at least one AME gene. The coexistence of two or three AME genes was detected in most gentamicin-resistant isolates. These results suggest an alarming rate of aminoglycoside resistance in this test location in Tehran, Iran. Continued surveillance at the genotypic and phenotypic levels, and adherence to well-designed antibiotic and infection-control policies are necessary to limit the spread of antimicrobial resistance.

Item Type: Article
Creators:
CreatorsEmail
Emaneini, M.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Eslampour, M. A.UNSPECIFIED
Sedaghat, H.UNSPECIFIED
Aligholi, M.UNSPECIFIED
Jabalameli, F.UNSPECIFIED
Shahsavan, S.UNSPECIFIED
Sotoudeh, N.UNSPECIFIED
Keywords: polymerase-chain-reaction modifying enzymes antimicrobial resistance multiplex pcr aureus genes hospitals susceptibility surveillance infections Infectious Diseases Microbiology Pharmacology & Pharmacy
Divisions:
Page Range: pp. 129-132
Journal or Publication Title: Microbial Drug Resistance
Journal Index: ISI
Volume: 15
Number: 2
Identification Number: https://doi.org/10.1089/mdr.2009.0869
ISSN: 1076-6294
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/891

Actions (login required)

View Item View Item