Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Associations of risk factors obesity and occupational airborne exposures with CDKN2A/p16 aberrant DNA methylation in esophageal cancer patients

(2010) Associations of risk factors obesity and occupational airborne exposures with CDKN2A/p16 aberrant DNA methylation in esophageal cancer patients. Diseases of the Esophagus. pp. 597-602. ISSN 1120-8694

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Abstract

P>It is known that obesity and occupational airborne exposure such as dust are among risk factors of esophageal cancer development, in particular squamous cell carcinoma (SCC) of esophagus. Here, we tested whether these factors could also affect aberrant DNA methylation. DNAs from 44 fresh tumor tissues and 19 non-tumor adjacent normal tissues, obtained from 44 patients affected by SCC of esophagus (SCCE), were studied for methylation at the CDKN2A/p16 gene promoter by methylation-specific polymerase chain reaction assay. Statistical methods were used to assess association of promoter methylation with biopathological, clinical, and personal information data, including obesity and airborne exposures. Methylation at the CDKN2A/p16 gene promoter was detected in 12 out of 44 tumor samples. None of the non-tumor tissues exhibited the aberrant methylation. Our results confirmed previously described significant association with low tumor stage (P = 0.002); in addition, we found that obesity (P = 0.001) and occupational exposure (P = 0.008) were both significantly associated with CDKN2A/p16 promoter methylation. This study provides evidence that obesity and occupational exposure increase the risk of developing esophageal cancer through an enhancement of CDKN2A/p16 promoter methylation.

Item Type: Article
Keywords: body mass index CDKN2A p16 DNA methylation esophageal cancer occupational exposure squamous-cell carcinoma tumor-suppressor gene promoter methylation construction workers hypermethylation adenocarcinoma inactivation island progression p16(ink4a) Gastroenterology & Hepatology
Page Range: pp. 597-602
Journal or Publication Title: Diseases of the Esophagus
Journal Index: ISI
Volume: 23
Number: 7
Identification Number: https://doi.org/10.1111/j.1442-2050.2010.01059.x
ISSN: 1120-8694
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/871

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