Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria

(2010) Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria. Asian Biomedicine. pp. 901-911. ISSN 1905-7415

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Abstract

Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles, and random amplified polymorphism DNA polymerase chain reaction (RAPD-PCR) typing. Methods: One hundred twelve Acinetobacter isolates (including 66 A. baumannii and 46 non-Acinetobacter baumannii strains) were obtained from three university hospitals. The source of infection of these isolates included blood, urine, wound, and respiratory tract. Their susceptibilities to 17 antibiotics were tested and then all Acinetobacter isolates were typed by plasmid analysis and RAPD-PCR method. Results: A. baumannii isolates revealed nine different patterns of antibiotic resistance. Of those, non-A. baumannii, were associated with plasmid and RAPD-PCR typings (p<0.05). A. baumannii was more resistant to multiple antibiotics than non-A. baumannii (p<0.05). Seven different plasmid profiles were observed among 1 12 Acinetobacter isolates. Plasmids were found in 107 (95.5) of the 112 isolates. Unlike in RAPD-PCR typing, there was no difference between the type of Acinetobacter, A. or non-A. baumannii strains and plasmid profiles (p>0.05). By RAPD-PCR, six profiles were found for each A. and non-A. baumannii strains. The pattern 6 was the most common pattern among the isolates. Both plasmid and RAPD-PCR typing showed no association between plasmid profiling and site of infection (p>0.05). Conclusion: There is a wide spread of multi-drug resistant Acinetobacter spp., particularly A. baumannii, in the Middle East region that can be traced efficiently by plasmid and genotyping typing of Acinetobacter More care should be taken for tracing the development of antimicrobial resistance of Acinetobacter using precise molecular typing techniques.

Item Type: Article
Keywords: Acinetobacter baumannii antimicrobial susceptibility molecular typing plasmid profiles RAPD-PCR carbapenem-resistant acinetobacter intensive-care-unit blood-stream infection molecular epidemiology risk-factors hong-kong baumannii outbreak sulbactam complex Research & Experimental Medicine
Divisions:
Page Range: pp. 901-911
Journal or Publication Title: Asian Biomedicine
Journal Index: ISI
Volume: 4
Number: 6
Identification Number: https://doi.org/10.2478/abm-2010-0118
ISSN: 1905-7415
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/863

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