Repository of Research and Investigative Information

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Ilam University of Medical Sciences

Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec Type III

Mon Oct 3 01:07:33 2022

(2011) Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec Type III. Polish Journal of Microbiology. pp. 303-307. ISSN 1733-1331

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Abstract

Methicillin resistant Staphylococcus aureus (M RSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF) profile among MRSA isolates, from patients in Tehran, was evaluated by PCR and Multiplex-PCR. The MLVF typing of 144 MRSA isolates with type III SCCmec produced 5 different MLVF types. Generally, 97.2 (140/144) of all the isolates were positive for at least one of the tested exotoxin genes. The most prevalent genes were hid, found in 87.5 (126/144) of the isolates followed by lukE-lukD and hla found in 72.9 (105/144) and 70.1 (101/144) of the isolates, respectively. The tst gene, belonging to MLVF types I, IV and V, was found among three of the isolates from blood and wound samples. The sea gene was detected in 58.3 (84/144) of the isolates and the sed and see genes were found in one isolate with MLVF type V. The coexistence of genes was observed in the 87.5 (126/144) of the isolates. The rate of coexistence of hid with lukE-lukD, hla with lukE-lukD and sea with lukE-lukD were 66.7 (96/144), 44.4 (641144) and 44.4 (64/144), respectively. The present study demonstrated that MRSA strains with type III SCCmec show different MLVF patterns and exotoxin profiles.

Item Type: Article
Creators:
CreatorsEmail
Emaneini, M.UNSPECIFIED
Jabalameli, L.UNSPECIFIED
Iman-Eini, H.UNSPECIFIED
Aligholi, M.UNSPECIFIED
Ghasemi, A.UNSPECIFIED
Nakhjavani, F. A.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Khoramian, B.UNSPECIFIED
Asadollahi, P.UNSPECIFIED
Jabalameli, F.UNSPECIFIED
Keywords: methicillin-resistant Staphylococcus aureus MLVF exotoxin exfoliative toxins genes strains enterotoxin prevalence superantigens association disease tehran mrsa Microbiology
Divisions:
Page Range: pp. 303-307
Journal or Publication Title: Polish Journal of Microbiology
Journal Index: ISI
Volume: 60
Number: 4
ISSN: 1733-1331
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/860

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