Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

SACCHAROMYCES CEREVISIAE, KEY ROLE OF MIG1 GENE IN METABOLIC SWITCHING: PUTATIVE FERMENTATION/OXIDATION

Thu Oct 6 07:58:59 2022

(2018) SACCHAROMYCES CEREVISIAE, KEY ROLE OF MIG1 GENE IN METABOLIC SWITCHING: PUTATIVE FERMENTATION/OXIDATION. Journal of Biological Regulators and Homeostatic Agents. pp. 649-654. ISSN 0393-974X

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Abstract

Saccharomyces cerevisiae can utilize a wide range of carbon sources; however, in the presence of glucose the use of alternate carbon sources would be repressed. Several genes involved in the metabolic pathways exert these effects. Among them, the zinc finger protein, Mig1 (multicopy inhibitor of GAL gene expression) plays important roles in glucose repression of Saccharomyces cerevisiae. To investigate whether the alleviation of glucose effect would result in a switch to oxidative production pathway, MIG1 were disrupted in a haploid laboratory strain (2805) of S. cerevisiae. The impact of this disruption was studied under fully aerobic conditions when glucose was the sole carbon source. Our results showed that glucose repression was partly alleviated; i.e., ethanol, as a significant fermentation marker, and acetate productions were respectively decreased by 14.13 and 43.71 compared to the wild type. In Delta MIG1 strain, the metabolic shifting on the aerobic pathway and a significant increase in pyruvate and glycerol production suggested it as an optimally productive industrial yeast strain. However, further studies are needed to confirm these findings.

Item Type: Article
Creators:
CreatorsEmail
Alipourfard, I.UNSPECIFIED
Bakhtiyari, S.UNSPECIFIED
Datukishvili, N.UNSPECIFIED
Haghani, K.UNSPECIFIED
Di Renzo, L.UNSPECIFIED
De Miranda, R. C.UNSPECIFIED
Cioccoloni, G.UNSPECIFIED
Yazdi, P. B.UNSPECIFIED
Mikeladze, D.UNSPECIFIED
Keywords: yeast ethanol metabolic engineering glucose repression aerobic pathway yeast Endocrinology & Metabolism Immunology Research & Experimental Medicine Physiology
Divisions:
Page Range: pp. 649-654
Journal or Publication Title: Journal of Biological Regulators and Homeostatic Agents
Journal Index: ISI
Volume: 32
Number: 3
ISSN: 0393-974X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/85

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