Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Phenotypic and Genotypic Assay for Detection of Extended Spectrum beta-lactamases Production by Klebsiella pneumoniae Isolates in Emam Reza Hospital in Tabriz, Iran

Sat Jun 25 09:30:46 2022

(2011) Phenotypic and Genotypic Assay for Detection of Extended Spectrum beta-lactamases Production by Klebsiella pneumoniae Isolates in Emam Reza Hospital in Tabriz, Iran. Journal of Pure and Applied Microbiology. pp. 1-10. ISSN 0973-7510

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Abstract

Objectives of this study were to investigate the prevalence of K. pneumoniae producing ESBLs, to evaluate the susceptibility of K. pneumoniae producing ESBLs towards non-beta-lactam antibiotics and to study the dominant ESBLs gene in Emam Reza hospital. K. pneumoniae producing ESBLs identified by phenotypic and genotypic methods. Polymerase Chain Reaction (PCR) performed for detection of blaSHV, TEM and CTX-M. The findings showed that 43.69, 13.59, 7.77, 11.65 and 23.3 were from UT!, ICUs, surgery ward, lesion infections and RTI, respectively. The results showed that 43.7 of isolates were ESBLs produces. The findings revealed that 26.7, 6.7, 20 and 0 of K.pneumoniae producing ESBLs were resistant to amikacin, ciprofloxacin, cotrimoxazol and imipenem, respectively. Thirty-nine blaSHV, seven blaTEM and seven blaCTX-M identified among K.pneumoniae producing ESBLs. The results reflected in cold month resistant to third generation cephalosporins were more than warm months. Generally, frequency of blaSHV was more than blaCTX-M and blaTEM.

Item Type: Article
Creators:
CreatorsEmail
Ghafourian, S.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Bin Sekawi, Z.UNSPECIFIED
Neela, V. K.UNSPECIFIED
Shamsudin, M. N.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Galehdari, E. A.UNSPECIFIED
Maleki, A.UNSPECIFIED
Pornour, M.UNSPECIFIED
Mobaiyen, H.UNSPECIFIED
Rahbar, M.UNSPECIFIED
Keywords: Phenotypic Genotypic K. pneumoniae and Tabriz bacteremia Biotechnology & Applied Microbiology Microbiology
Divisions:
Page Range: pp. 1-10
Journal or Publication Title: Journal of Pure and Applied Microbiology
Journal Index: ISI
Volume: 5
Number: 1
ISSN: 0973-7510
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/847

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