Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM RAW MILK OF BOVINE SUBCLINICAL MASTITIS IN TEHRAN AND MASHHAD

Sat Jun 25 09:26:22 2022

(2011) CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS STRAINS ISOLATED FROM RAW MILK OF BOVINE SUBCLINICAL MASTITIS IN TEHRAN AND MASHHAD. Acta Microbiologica Et Immunologica Hungarica. pp. 113-121. ISSN 1217-8950

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Abstract

Staphylococcus aureus is considered one of the most important food borne pathogens. A total of 111 isolates of S. aureus were cultured from raw milk samples during January 2009 to June 2009 from Tehran and Mashhad. The coagulase gene polymorphism and the prevalence of classical enterotoxin genes of S. aureus strains were determined by PCR-RFLP (restriction fragment length polymorphism) and Multiplex-PCR. Disk diffusion method was used to determine the susceptibility of isolates to antimicrobial agents as instructed by Clinical and Laboratory Standards Institute. Sixty-seven of the isolates harboured one or more enterotoxin genes. The most prevalent gene was sec, found in 59 of the isolates. Approximately 8 of the isolates were positive for sea, seb and sed genes. Only one isolate had see gene. The rate of coexistence of enterotoxin genes was 14. All S. aureus isolates were susceptible to ciprofloxacin, gentamicin, imipenem, minocycline, oxacillin and vancomycin. They were resistant to ampicillin (64), penicillin (56), clindamycin (22), tetracycline (22), doxycycline (19), teicoplanin (13), rifampin (2) and tri-methoprim-sulfamethoxazole (2). On the basis of coagulase gene analysis of 111 S. aureus isolates, the PCR products of 56 isolates were digested with Alu I that produced three distinct patterns. These data indicate the high prevalence of enterotoxigenic S. aureus in raw bovine milk in Tehran and Mashhad, and highlight the importance of proper quality control of dairy products for public health.

Item Type: Article
Creators:
CreatorsEmail
Sahebekhtiari, N.UNSPECIFIED
Nochi, Z.UNSPECIFIED
Eslampour, M. A.UNSPECIFIED
Dabiri, H.UNSPECIFIED
Bolfion, M.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Khoramian, B.UNSPECIFIED
Zali, M. R.UNSPECIFIED
Emaneini, M.UNSPECIFIED
Keywords: Staphylococcus aureus raw bovine milk enterotoxin gene coagulase gene Iran coagulase gene polymorphism field gel-electrophoresis food poisoning outbreaks antimicrobial susceptibility pcr detection enterotoxins antibiogram genotypes cows Immunology Microbiology
Divisions:
Page Range: pp. 113-121
Journal or Publication Title: Acta Microbiologica Et Immunologica Hungarica
Journal Index: ISI
Volume: 58
Number: 2
Identification Number: https://doi.org/10.1556/AMicr.58.2011.2.4
ISSN: 1217-8950
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/845

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