Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) and antibacterial resistance profiles of extended spectrum beta lactamase (ESBL) producing Pseudomonas aeruginosa among burnt patients in Tehran

Sat Jun 25 10:02:09 2022

(2011) Multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) and antibacterial resistance profiles of extended spectrum beta lactamase (ESBL) producing Pseudomonas aeruginosa among burnt patients in Tehran. Burns. pp. 1202-1207. ISSN 0305-4179

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Abstract

Extended spectrum p-lactamase (ESBL)-producing trait was present in 48 out of the 112 (42.8) Pseudomonas aeruginosa isolates collected from burn wound infections during a 12-month period. The presence of oxa-10, per-1, veb-1 and ges genes and the multiple-locus variable number of tandem repeats (VNTR) fingerprinting (MLVF) of 112 P. aeruginosa strains were determined by PCR and multiplex PCR. Disk diffusion methods were used to determine the susceptibility of the isolates to antimicrobial agents as instructed by CLSI. All ESBL isolates were resistant to aztreonam, cefepime, cefotaxime, cefpodoxime, ceftazidime, ceftriaxone and ofloxacin. Fewer than 60 of ESBL isolates were resistant to imipenem, meropenem, and piperacillin-tazobactam but more than 90 were resistant to amikacin, ciprofloxacin, levofloxacin, ticarcillin and tobramycin. The most prevalent ESBL genes included oxa-10 (70) and per-1 (50) followed by veb-1 (31.3). The gene encodes GES enzyme did not detect in any isolates. A total of 100 P. aeruginosa strains were typed by MLVF typing method. MLVF produced 42 different DNA banding patterns. These data indicate that different MLVF types infect burn wounds in patients at a hospital in Tehran and also suggest an alarming rate of ESBL-producing isolates in this test location. (C) 2011 Elsevier Ltd and ISBI. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Jabalameli, F.UNSPECIFIED
Mirsalehian, A.UNSPECIFIED
Sotoudeh, N.UNSPECIFIED
Jabalameli, L.UNSPECIFIED
Aligholi, M.UNSPECIFIED
Khoramian, B.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Emaneini, M.UNSPECIFIED
Keywords: Pseudomonas aeruginosa VNTR ESBL Burn ambler class-a molecular epidemiology clinical impact turkey unit infection prevalence outbreak istanbul genes General & Internal Medicine Dermatology Surgery
Divisions:
Page Range: pp. 1202-1207
Journal or Publication Title: Burns
Journal Index: ISI
Volume: 37
Number: 7
Identification Number: https://doi.org/10.1016/j.burns.2011.05.012
ISSN: 0305-4179
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/818

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