Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus

(2012) Evaluation of rabbit antibody response against 8 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus. Asian Pacific Journal of Tropical Medicine. pp. 355-357. ISSN 1995-7645

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Abstract

Objective: To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B front Echinococcus granulosus (E. granulosus) and measuring polyelonal antibody and Immoral immune response using ELISA and gel diffusion. Methods: Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography. Four young rabbits were selected and challenged intradermally with yielded recombinant antigens. Rabbits' sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection. Results: The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection. Conclusions: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.

Item Type: Article
Keywords: Cystic echinococcosis Hydatidosis Polyclonal antibody linked-immunosorbent-assay cystic hydatid-disease serological evaluation immunodiagnosis expression cloning update fluid mice Public, Environmental & Occupational Health Tropical Medicine
Page Range: pp. 355-357
Journal or Publication Title: Asian Pacific Journal of Tropical Medicine
Journal Index: ISI
Volume: 5
Number: 5
Identification Number: https://doi.org/10.1016/s1995-7645(12)60058-3
ISSN: 1995-7645
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/791

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