Abstract

Klebsiella pneumoniae is a common hospital-acquired pathogen, causing urinary tract infections, nosocomial pneumoniae, and intra-abdominal infections. K.pneumoniae is also a potential community acquired pathogen. The aims of this study are to determine epidemiology of ESBL-producing K.pneumoniae and K.oxytoca in Iran during different seasons, to determine the prevalence of blaTEM, SHY and CTX-M responsible for ESBL production among ESBL-producing K.pneumoniae and K.oxytoca in the different wards and hospitals in Iran. Klebsiella spp producing ESBLs were identified by phenotypic and genotyping methods. The findings in this study revealed that 36.5, 51.7 and 45.6 of K.pneumoniae were producing ESBLs in Ilam, Milad and Emam Reza hospitals, respectively. The results revealed blaSHV was responsible for ESBLs production. The analysis showed significant difference of ESBLs production by K.pneumoniae in winter (53) in comparison to the other seasons P <= 0.01. The results also showed a significant difference in blaSHV that was the dominant gene responsible for ESBL production P <= 0.049 but no significant difference was observed between blaTEM and blaSHV. The results showed that the highest ESBL production was found in K.oxytoca isolated from patients in Emam Reza Hospital and the lowest frequency of ESBL production was found among K.oxytoca in Ham hospital. BlaSHV was found as dominant gene responsible for ESBLs production by K.pneumoniae and K.oxytoca, followed by blaCTX-M.