Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

High Expression of FOXP3 mRNA in Blood and Urine as a Predictive Marker in Kidney Transplantation

Sat Dec 10 02:59:52 2022

(2018) High Expression of FOXP3 mRNA in Blood and Urine as a Predictive Marker in Kidney Transplantation. Progress in Transplantation. pp. 134-141. ISSN 1526-9248

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Abstract

Background: Diagnosis of allograft dysfunction by noninvasive biomarker tests is preferable to invasive allograft biopsies and has been extensively considered in recent years. This study aims to evaluate blood and urinary forkhead box P3 (FOXP3) messenger RNA (mRNA) expression in renal transplant recipients in an attempt to determine whether differential diagnosis of graft dysfunction is feasible using mRNA profiles. Methods: We analyzed FOXP3 mRNA expression in paired urinary and peripheral blood mononuclear cell (PBMC) samples. A total of 91 kidney transplant recipients enrolled in this study that were classified into 3 groups: biopsy-proven acute rejection (AR; n = 27), chronic allograft nephropathy (n = 19), and well-functioning graft (n = 45). The FOXP3 mRNA expression was quantified by TaqMan probe real-time polymerase chain reaction. Results: Acute rejection patients had a higher expression level of transcription factor FOXP3 compared to the chronic nephropathy and control groups. Analysis of receiver operating characteristic curves showed that rejection could be diagnosed with 100 sensitivity and 96 specificity in urine, and 92 sensitivity and 86 specificity in PBMC samples using the optimal FOXP3 mRNA cutoff value. We subdivided the AR group into progressive and nonprogressive patients, which showed a significant difference in FOXP3 mRNA expression. This result confirmed the role of FOXP3 as a diagnostic marker in predicting transplantation outcomes. Conclusion: Our results suggested that elevated expression of FOXP3 in blood and urine samples from kidney transplant recipients could be a useful noninvasive biomarker to diagnose graft dysfunction.

Item Type: Article
Creators:
CreatorsEmail
Barabadi, M.UNSPECIFIED
Shahbaz, S. K.UNSPECIFIED
Foroughi, F.UNSPECIFIED
Hosseinzadeh, M.UNSPECIFIED
Nafar, M.UNSPECIFIED
Yekaninejad, M. S.UNSPECIFIED
Amirzargar, A.UNSPECIFIED
Keywords: FOXP3 expression urine blood kidney transplantation regulatory t-cells antibody-mediated rejection acute cellular rejection renal-allografts gene-expression graft function recipients biopsies induction tolerance Surgery Transplantation
Divisions:
Page Range: pp. 134-141
Journal or Publication Title: Progress in Transplantation
Journal Index: ISI
Volume: 28
Number: 2
Identification Number: https://doi.org/10.1177/1526924818765812
ISSN: 1526-9248
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/77

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