Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Characterization of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel Electrophoresis

(2012) Characterization of Alloiococcus otitidis strains isolated from children with otitis media with effusion by Pulsed-Field Gel Electrophoresis. International Journal of Pediatric Otorhinolaryngology. pp. 1658-1660. ISSN 0165-5876

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Abstract

Objective: Alloiococcus otitidis is a slow growing organism which has been isolated in a few studies on patients with otitis media with effusion (OME). According to the literature review, there is no study about the molecular typing of A. otitidis. In this study, the characteristics of A. otitidis isolates from patients with OME were investigated via Pulsed-Field Gel Electrophoresis (PFGE) typing method. Methods: A total of 50 children with OME, who underwent myringotomy or who had an insertion of a ventilation tube, were included in this study. The isolates were identified to the species level as A. otitidis using standard biochemical methods, following which the amplification and sequencing of the 16S rRNA gene were carried out. The molecular characteristic of A. otitidis was investigated by PFGE technique. Results: Fifteen isolates of A. otitidis were identified in the middle ear fluid of the patients. All the isolates were susceptible to ampicillin, amoxicillin/clavulanate and fluoroquinolones. All of the 15 isolates were typed by PFGE method and were found to include 13 different PFGE types. Conclusion: The current study, being the first reports on the molecular typing of A. otitidis by PFGE method, shows that A. otitidis is a heterogenic organism in Iranian children who have OME. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Keywords: Alloiococcus otitidis Pulsed-Field Gel Electrophoresis (PFGE) Otitis media middle-ear fluid molecular analysis pathogens organism Otorhinolaryngology Pediatrics
Page Range: pp. 1658-1660
Journal or Publication Title: International Journal of Pediatric Otorhinolaryngology
Journal Index: ISI
Volume: 76
Number: 11
Identification Number: https://doi.org/10.1016/j.ijporl.2012.07.040
ISSN: 0165-5876
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/768

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