Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

SURVEY IN IRAN OF CLARITHROMYCIN RESISTANCE IN HELICOBACTER PYLORI ISOLATES BY PCR-RFLP

Sun Aug 14 02:17:20 2022

(2013) SURVEY IN IRAN OF CLARITHROMYCIN RESISTANCE IN HELICOBACTER PYLORI ISOLATES BY PCR-RFLP. Southeast Asian Journal of Tropical Medicine and Public Health. pp. 89-95. ISSN 0125-1562

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

The aims of this study were to assess primary resistance of H. pylori strains isolated from adult patients of Ilam, Iran to antibacterial agents (amoxicillin, clarithromycin, metronidazole and tetracycline) and detection of clarithromycin, azithromycin, clarithromycin, metronidazole and tetracycline resistance by disc diffusion. Fifty biopsies were taken from gastric mucosa of the antrum and body regions of adult patients by gastroscopy, and were cultured on Helicobacter pylori selective medium. The susceptibility of H. pylon strains showed that 44, 6, 6, 4 and 16 were resistance to metronidazole, amoxicillin, tetracycline, azithromycin, and clarithromycin, respectively. Polymerase chain reaction-restriction fragment length polymorphism analysis showed that all clarithromycin resistance isolates had A2143G mutation and PCR amplicons from these strains upon digestion by BsaI restriction enzyme resulted in 319 and 106 base pair fragments. Because most of physicians in Ilam do not use amoxicillin in triple therapy of H. pylon infection, isolates showed low rate of resistance to amoxicillin

Item Type: Article
Creators:
CreatorsEmail
Sadeghifard, N.UNSPECIFIED
Seidnazari, T.UNSPECIFIED
Ghafourian, S.UNSPECIFIED
Soleimani, M.UNSPECIFIED
Maleki, A.UNSPECIFIED
Qomi, M. A.UNSPECIFIED
Sekawi, Z.UNSPECIFIED
Keywords: clarithromycin resistance H.pylori PCR-RFLP Iran 23s ribosomal-rna antibiotic-resistance korean patients mutations strains susceptibilities metronidazole hybridization amoxicillin prevalence Public, Environmental & Occupational Health Infectious Diseases Tropical Medicine
Divisions:
Page Range: pp. 89-95
Journal or Publication Title: Southeast Asian Journal of Tropical Medicine and Public Health
Journal Index: ISI
Volume: 44
Number: 1
ISSN: 0125-1562
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/753

Actions (login required)

View Item View Item