Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier

Wed Nov 30 16:25:36 2022

(2013) Soluble Flt-1 Gene Delivery in Acute Myeloid Leukemic Cells Mediating a Nonviral Gene Carrier. Biomed Research International. p. 8. ISSN 2314-6133

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Abstract

Vascular endothelial growth factor (VEGF) is a potent angiogenic factor involved in angiogenesis-mediated progression of acute myeloid leukemia (AML). Studies have reported the role of soluble form of fms-like tyrosine kinase (sFlT-1) delivery as an antitumor agent by inhibiting VEGF. is study investigates the outcome of delivery of a VEGF165 antagonist, soluble vascular endothelial growth factor receptor, namely sFLT-1, mediating lipofectamine 2000 in acute myeloid leukemic cells. A recombinant plasmid expressing sFLT-1 was constructed and transfected into the K562 and HL60 cells using lipofectamine 2000 transfection reagent. sFLT-1 expression/secretion in pVAX-sFLT-1 transfected cells was verified by RT-PCR and western blot. MTS assay was carried out to evaluate the effect of sFLT-1 on human umbilical vein endothelial cells and K562 and HL60 cells in vitro. Treatment with pVAX-sFLT-1 showed no association between sFLT-1 and proliferation of infected K562 and HL60 cells, while it demonstrated a significant inhibitory impact on the proliferation of HUVECs. The results of the current study imply that the combination of nonviral gene carrier and sFLT-1 possesses the potential to provide efficient tool for the antiangiogenic gene therapy of AML.

Item Type: Article
Creators:
CreatorsEmail
Amini, R.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Veerakumarasivam, A.UNSPECIFIED
Abdullah, S.UNSPECIFIED
Abdulamir, A. S.UNSPECIFIED
Nadali, F.UNSPECIFIED
Rosli, R.UNSPECIFIED
Keywords: endothelial growth-factor factor receptor factor vegf increased angiogenesis extracellular domain tumor-growth in-vivo expression therapy mechanisms Biotechnology & Applied Microbiology Research & Experimental Medicine
Divisions:
Page Range: p. 8
Journal or Publication Title: Biomed Research International
Journal Index: ISI
Identification Number: https://doi.org/10.1155/2013/752603
ISSN: 2314-6133
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/752

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