Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Genetic similarity between adenoid tissue and middle ear fluid isolates of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis from Iranian children with otitis media with effusion

Sun Aug 14 01:21:32 2022

(2013) Genetic similarity between adenoid tissue and middle ear fluid isolates of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis from Iranian children with otitis media with effusion. International Journal of Pediatric Otorhinolaryngology. pp. 1841-1845. ISSN 0165-5876

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Abstract

Background: Otitis media with effusion (OME) is a common disease among children, in the pathogenesis of which bacterial infections play a critical role. It was suggested that adenoid tissue could serve as a reservoir for bacterial infection, the eustachian tubes being the migration routes of bacteria into the middle ear cavity. The aim of this study was to investigate the genetic similarity between isolates of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis, obtained from adenoid tissue and middle ear fluid. Methods: A total of 60 specimens of middle ear fluids (MEFs) and 45 specimens of adenoid tissue were obtained from 45 children with OME. All the samples were inoculated on culture media for bacterial isolation and identification. The genetic similarity between bacterial isolates was determined by pulsed-field gel electrophoresis (PFGE). Results: The same bacterial species were simultaneously isolated from adenoid tissue and MEFs of 14 patients, among which, 6 pairs of M. catarrhalis, 5 pairs of S. pneumoniae and 3 pairs of H. influenzae were identified. Conclusions: Based on the genetic similarities between isolate pairs, found by PFGE analysis, this study suggested that M. catarrhalis, S. pneumoniae and H. influenzae colonize the adenoid tissue, then migrate to the middle ear cavity and, hence, contribute to the total pathogenesis of OME. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Emaneini, M.UNSPECIFIED
Gharibpour, F.UNSPECIFIED
Khoramrooz, S. S.UNSPECIFIED
Mirsalehian, A.UNSPECIFIED
Jabalameli, F.UNSPECIFIED
Darban-Sarokhalil, D.UNSPECIFIED
Mirzaii, M.UNSPECIFIED
Sharifi, A.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Keywords: Otitis media with effusion Streptococcus pneumoniae Moraxella catarrhalis Haemophilus influenzae Pulsed-field gel electrophoresis (PFGE) nontypable hemophilus-influenzae field gel-electrophoresis outer-membrane protein alloiococcus-otitidis resistant-bacteria nasopharyngeal childhood recovery disease flora Otorhinolaryngology Pediatrics
Divisions:
Page Range: pp. 1841-1845
Journal or Publication Title: International Journal of Pediatric Otorhinolaryngology
Journal Index: ISI
Volume: 77
Number: 11
Identification Number: https://doi.org/10.1016/j.ijporl.2013.08.024
ISSN: 0165-5876
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/699

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