Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Metabolic engineering of Lactococcus lactis influence of the overproduction of lipase enzyme

Mon May 20 16:05:34 2024

(2013) Metabolic engineering of Lactococcus lactis influence of the overproduction of lipase enzyme. Journal of Dairy Research. pp. 490-495. ISSN 0022-0299

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The dairy industry uses lipase extensively for hydrolysis of milk fat. Lipase is used in the modification of the fatty acid chain length, to enhance the flavours of various chesses. Therefore finding the unlimited source of lipase is a concern of dairy industry. Due to the importance of lipase, this study was an attempt to express the lipase from Burkholderia cepacia in Lactococcus lactis. To achieve this, a gene associated with lipase transport was amplified and subcloned in inducible pNZ8148 vector, and subsequently transformed into Lc. lactis NZ9000. The enzyme assay as well as SDS-PAGE and western blotting were carried out to analysis the recombinant lipase expression. Nucleotide sequencing of the DNA insert from the clone revealed that the lipase activity corresponded to an open reading frame consisting of 1092 bp coding for a 37.5-kDa size protein. Blue colour colonies on nile blue sulphate agar and sharp band on 37.5-kD size on SDS-PAGE and western blotting results confirm the successful expression of lipase by Lc. lactis. The protein assay also showed high expression, approximately 152.2 mu g/ml.h, of lipase by recombinant Lc. lactis. The results indicate that Lc. lactis has high potential to overproduce the recombinant lipase which can be used commercially for industrially purposes.

Item Type: Article
Keywords: Lactococcus lactis lipase overproduction controlled gene-expression acid bacteria escherichia-coli system nice heterologous proteins bacillus-subtilis nisin purification secretion biotechnology Agriculture Food Science & Technology
Page Range: pp. 490-495
Journal or Publication Title: Journal of Dairy Research
Journal Index: ISI
Volume: 80
Number: 4
Identification Number:
ISSN: 0022-0299
Depositing User: مهندس مهدی شریفی

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