Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Trehalose Activates Autophagy and Prevents Hydrogen Peroxide-Induced Apoptosis in the Bone Marrow Stromal Cells

Mon May 20 16:53:02 2024

(2018) Trehalose Activates Autophagy and Prevents Hydrogen Peroxide-Induced Apoptosis in the Bone Marrow Stromal Cells. Iranian Journal of Pharmaceutical Research. pp. 1141-1149. ISSN 1735-0328

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Bone marrow stromal stem cells (BMSCs) play a significant role in cell therapy. These cells quickly die after transplantation to the affected area due to oxidative stress. The natural disaccharide, trehalose which can be known as autophagy inducer. The present study aimed to investigate the role of trehalose in preventing BMSCs from oxidative stress caused by H2O2. BMSCs were isolated from the adult rats. The cells were divided into three groups: (a) control; (b) 100 mu M H2O2; (c) 100 mu M H2O2 and trehalose 3. The morality rate was analyzed by viability test. Immunocytochemistry and Western blot was used in order to evaluate p62 protein and LC3II/LC3I ratio, respectively. In order to evaluate apoptosis, cleaved caspase-3 protein was used. In viability test, the survival rate for BMSCs after 8 h were 82, 72, 49, and 39 (for groups who received 50, 100, 200, and 400 mu M H2O2, respectively) compared to the control group. Pre-treatment with the use of trehalose 3 increased cell survivals. The levels of p62 protein, were increased in the cells under H2O2 treatment, while the levels of p62 protein in the cytoplasm, as autophagy inclusions, reduced for the group with trehalose pre-treatment. In addition, trehalose caused to increase LC3II/LC3I ratio and decreased the expression of cleaved caspase-3. Trehalose decreased apoptosis and increased the autophagy and survival levels of the cells against H2O2. Due to the unique properties of trehalose and its low toxicity, it can be used as a pharmaceutical agent in cellular transplantation to reduce oxidative stress.

Item Type: Article
Abbaszadeh, H. A.UNSPECIFIED
Keywords: Stress oxidative Autophagy Apoptosis Bone marrow stromal cells Trehalose mesenchymal stem-cells mouse model stress death dysfunction induction hypoxia disease complex growth Pharmacology & Pharmacy
Page Range: pp. 1141-1149
Journal or Publication Title: Iranian Journal of Pharmaceutical Research
Journal Index: ISI
Volume: 17
Number: 3
ISSN: 1735-0328
Depositing User: مهندس مهدی شریفی

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