Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

The Prevalence of Pneumocystis jiroveci in Bronchoalveolar Lavage Specimens of Lung Transplant Recipients Examined by the Nested PCR

Mon Aug 8 17:53:40 2022

(2014) The Prevalence of Pneumocystis jiroveci in Bronchoalveolar Lavage Specimens of Lung Transplant Recipients Examined by the Nested PCR. Jundishapur Journal of Microbiology. p. 4. ISSN 2008-3645

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Abstract

Background: The use of immune suppressive drugs for organ transplant recipients predisposes them to opportunistic infections, especially by fungal agents. Pneumocystis jiroveci, as an opportunistic pathogen, endangers the patients' life in those with immune system disorders. Early detection of latent Pneumocystis infection in susceptible patients may help choose the optimal treatment for these patients. Objectives: The aim of this study was to identify and determine the colonization of latent P. jiroveci infection among lung transplant recipients. Patients and Methods: This cross-sectional descriptive study was conducted on lung transplant recipients. Bronchoalveolar lavage (BAL) specimens were collected from 32 patients undergoing bronchoscopy. The samples were aseptically homogenized by 10 mM dithiothreitol, and their DNA was extracted. The mtLSUrRNA gene of P. jiroveci was amplified using nested PCR in two stages. Nested PCR was performed using external primers of pAZ-102-E and pAZ102-H followed by using the PCR product of the first stage and internal primers of pAZ-102-E and pAZ102-L2. Results: The genome of P. jiroveci was revealed by a 346 bp PCR product in the initial amplification and a 120 bp product in the nested PCR. The results showed that seven BAL specimens (21.9) from lung transplant recipients were positive for P. jiroveci. Conclusions: In molecular epidemiology studies, nested PCR has higher sensitivity than PCR. Results of this study support the colonization of P. jiroveci in patients receiving lung transplantation. Patients who are carriers of P. jiroveci are at a higher risk of P. jiroveci pneumonia.

Item Type: Article
Creators:
CreatorsEmail
Izadi, M.UNSPECIFIED
Jafari, N. J.UNSPECIFIED
Sadraei, J.UNSPECIFIED
Poornaki, A. M.UNSPECIFIED
Rezavand, B.UNSPECIFIED
Zarrinfar, H.UNSPECIFIED
Abdi, J.UNSPECIFIED
Mohammadi, Y.UNSPECIFIED
Keywords: Pneumocystis jiroveci Lung Transplantation Nested PCR Bronchoalveolar Lavage carinii-pneumonia immune-system DNA samples amplification prophylaxis infection drugs risk Microbiology
Divisions:
Page Range: p. 4
Journal or Publication Title: Jundishapur Journal of Microbiology
Journal Index: ISI
Volume: 7
Number: 12
Identification Number: https://doi.org/10.5812/jjm.13518
ISSN: 2008-3645
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/625

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