Repository of Research and Investigative Information

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Ilam University of Medical Sciences

Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein From Streptococcus pyogenes

Fri Mar 29 16:24:36 2024

(2015) Overexpression and Enzymatic Assessment of Antigenic Fragments of Hyaluronidase Recombinant Protein From Streptococcus pyogenes. Jundishapur Journal of Microbiology. p. 6. ISSN 2008-3645

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Abstract

Background: Hyaluronidase catalyzes the hydrolysis of hyaluronan polymers to N-acetyl-D-glucosamine and D-glucuronic acid. This enzyme is a dimer of identical subunits. Hyaluronidase has different pharmaceutical and medical applications. Previously, we produced a recombinant hyaluronidase antigenic fragment of Streptococcus pyogenes. Objectives: This study aimed to improve the protein production and purity of hyaluronidase recombinant protein from S. pyogenes. In addition, the enzymatic activity of this protein was investigated. Materials and Methods: The expression of hyaluronidase antigenic fragments was optimized using IPTG concentration, time of induction, temperature, culture, and absorbance of 0.6-0.8-1 at 600 nm. Afterwards, the expressed proteins were purified and the enzymatic activity was assessed by turbid metric method. Results: Data indicated that maximum protein is produced in OD = 0.8, 0.5 mM Isopropyl beta-D-1-thiogalactopyranoside (IPTG), 37 degrees C, NB 1.5x, without glucose, incubated for overnight. The enzymatic activity of the recombinant protein was similar to the commercial form of hyaluronidase. Conclusions: The results showed that an antigenic fragment of the recombinant hyaluronidase protein from S. pyogenes has a considerable enzymatic activity. It can be suggested to use it for medical purposes. In addition, applications of bioinformatics software would facilitate the production of a smaller protein with same antigenic properties and enzymatic activity.

Item Type: Article
Creators:
CreatorsEmail
Abbasian, S. S.UNSPECIFIED
Rad, E. G.UNSPECIFIED
Akbari, N.UNSPECIFIED
Zolfaghari, M. R.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Abtahi, H.UNSPECIFIED
Keywords: Hyaluronidase Optimization of Expression Enzymatic Activity escherichia-coli assay venom degradation sensitivity inhibitors virulence cancer Microbiology
Divisions:
Page Range: p. 6
Journal or Publication Title: Jundishapur Journal of Microbiology
Journal Index: ISI
Volume: 8
Number: 1
Identification Number: https://doi.org/10.5812/jjm.13653
ISSN: 2008-3645
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/606

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