Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Preventive efficacy evaluation of Urtica dioica on in vitro fertilization and embryo development in exposed mice

Tue Jul 16 16:44:51 2024

(2018) Preventive efficacy evaluation of Urtica dioica on in vitro fertilization and embryo development in exposed mice. Medical Science. pp. 431-438. ISSN 2321-7359

Full text not available from this repository.

Official URL:


Nowadays impact of cell-phone RF on human health is subject of many researches. Antioxidant properties of hydroalcoholic extract of Urtica dioica (UD) have been proven to eliminate free radicals and oxidative stress. Purpose of this study was to assess the impacts of cell-phone RF on in-vitro fertility (IVF) rate and role of the UD extract against induced the RF damages. A total number of 54 adult, male NMRI mice were randomly divided into six equal groups randomly; control group, RF-irradiation alone (2hrs a day for 28days), those received only 50mg/kg UD, 50mg/kg UD plus RF-irradiation, only 100mg/kg UD, and 100mg/kg UD plus RFirradiation. The UD was intraperitoneally injected 1hr before RF-irradiation. IVF performed 1day after the last RF-irradiation. Chromatin integrity in sperm DNA evaluated by acridine orange staining. Finally, frequency and morphology of the embryos were assessed. ANOVA and Tukey's tests applied for statistical analysis. RF-irradiation alone significantly decreased frequency of two-cell embryos (p=0.000) and grade-A embryos (p=0.014); while it significantly increased the dead embryos (p=0.001) and grade-B (p=0.033), grade-C (p=0.017) and grade-D embryos (p=0.008). RF-irradiation alone led to significant genetically damages in DNA chromatin (p=0.000). Injection of 100mg/kg UD increased significantly frequency of the two-cell embryos (p=0.01) and grade-A embryos (p=0.018); where it significantly reduced the dead embryos (p=0.026), grade-C (p=0.026) and grade-D embryos (p= 0.038). Cell-phone RF can change number of two-cell mouse embryo as well as embryo's morphology and induces molecular damages in the DNA of the sperm. Moreover, UD extract plays a protective role against RF induced damages.

Item Type: Article
Rostamzadeh, A.UNSPECIFIED
Keywords: Radiofrequency Urtica dioica Embryo Morphology Antioxidant mobile-phone electromagnetic-field prostatic hyperplasia antioxidant activity oxidative stress male-infertility cellular phone brain rats radiation Research & Experimental Medicine
Page Range: pp. 431-438
Journal or Publication Title: Medical Science
Journal Index: ISI
Volume: 22
Number: 92
ISSN: 2321-7359
Depositing User: مهندس مهدی شریفی

Actions (login required)

View Item View Item