Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Rosiglitazone, but Not Epigallocatechin-3-Gallate, Attenuates the Decrease in PGC-1 alpha Protein Levels in Palmitate-Induced Insulin-Resistant C2C12 Cells

Sat Jun 25 02:40:29 2022

(2015) Rosiglitazone, but Not Epigallocatechin-3-Gallate, Attenuates the Decrease in PGC-1 alpha Protein Levels in Palmitate-Induced Insulin-Resistant C2C12 Cells. Lipids. pp. 521-528. ISSN 0024-4201

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Abstract

Alteration of lipid metabolism is an important mechanism for the treatment of insulin resistance. PGC-1 alpha, a key regulator of mitochondrial biogenesis and function, plays an important role in the improvement of insulin sensitivity by increasing fatty acids beta-oxidation. In the present study, the effects of epigallocatechin-3-gallate (EGCG), an anti-obesity agent and enhancer of lipid catabolism, on PGC-1 alpha protein expression was examined and compared with anti-diabetic drug rosiglitazone (RGZ). After differentiation of C2C12 myoblasts to myotubes, insulin resistance was induced by palmitate treatment. Then the expression of the PGC-1a gene and glucose uptake were evaluated before and after treatment with RGZ and EGCG. Palmitate treatment significantly decreased PGC-1 alpha protein expression in C2C12 cells (P < 0.05). RGZ could restore the expression of PGC-1 alpha in palmitate treated cells (P > 0.05), while EGCG had no significant effect on the expression of this gene (P < 0.05). RGZ and EGCG significantly improved glucose uptake (by 2- and 1.54-fold, respectively) in myotubes treated with palmitate. These data suggest that RGZ and EGCG both exert their anti-diabetic activity by increasing insulin sensitivity, but with different molecular mechanisms. This effect of RGZ, unlike EGCG, is mediated, at least partly, by increasing PGC-1 alpha protein expression.

Item Type: Article
Creators:
CreatorsEmail
Karimfar, M. H.UNSPECIFIED
Haghani, K.UNSPECIFIED
Babakhani, A.UNSPECIFIED
Bakhtiyari, S.UNSPECIFIED
Keywords: Insulin resistance C2C12 muscle cells PGC-1 alpha gene Epigallocatechin-3-Gallate Rosiglitazone high-fat diet ameliorates glucose-tolerance obesity-related inflammation type-2 diabetes-mellitus skeletal-muscle cells green tea polyphenol ppar-gamma agonists mitochondrial biogenesis in-vivo triglyceride content Biochemistry & Molecular Biology Nutrition & Dietetics
Divisions:
Page Range: pp. 521-528
Journal or Publication Title: Lipids
Journal Index: ISI
Volume: 50
Number: 6
Identification Number: https://doi.org/10.1007/s11745-015-4016-x
ISSN: 0024-4201
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/574

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