Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Sewage as a rich source of phage study against Pseudomonas aeruginosa PAO

Sat Jun 25 03:42:00 2022

(2015) Sewage as a rich source of phage study against Pseudomonas aeruginosa PAO. Biologicals. pp. 238-241. ISSN 1045-1056

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Abstract

Pseudomonas aeruginosa is a ubiquitous organism which has emerged as a major public health threat in hospital environments. Overuse of antibiotics has significantly exacerbated the emergence of multi-drug resistant bacteria such as P. aeruginosa. Phages are currently being utilized successfully for aquaculture, agriculture and veterinary applications. The aim of this study was to isolate and characterize of lytic P. aeruginosa phage from sewage of Ilam, Iran. Phage was isolated from sewage that was added to the enrichment along with the host and subsequently filtered. Plaque assay was done by using an overlay method (also called the double agar layer method). Purified plaques were then amplified for characterization. Finally, RAPD-PCR method was conducted for genotyping and Transition electron micrograph (TEM) recruited to determine the morphology and phage family. The phage had high concentration and tremendous effects against a variety of clinical and general laboratory strains (ATCC15693) of P. aeruginosa. Among a set of primers in RAPD panel, only P2 and RAPD5 primers, were useful in differentiating the phages. TEM images revealed that the isolated phages were members of the Siphoviridae family. The phage effectiveness and specificity towards target bacteria and potential to control biofilm formations will be investigate in our further studies. (C) 2015 The International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Azizian, R.UNSPECIFIED
Nasser, A.UNSPECIFIED
Askari, H.UNSPECIFIED
Kalani, M. T.UNSPECIFIED
Sadeghifard, N.UNSPECIFIED
Pakzad, I.UNSPECIFIED
Amini, R.UNSPECIFIED
Nejad, A. S. M.UNSPECIFIED
Jalilian, F. A.UNSPECIFIED
Keywords: Bacteriophage Pseudomonas aeruginosa Lytic phage Phage therapy TEM RAPD-PCR staphylococcus-aureus escherichia-coli therapy bacteriophages resistance diversity biofilm Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Pharmacology & Pharmacy
Divisions:
Page Range: pp. 238-241
Journal or Publication Title: Biologicals
Journal Index: ISI
Volume: 43
Number: 4
Identification Number: https://doi.org/10.1016/j.biologicals.2015.05.004
ISSN: 1045-1056
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/567

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