Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Low prevalence of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from a tertiary burn care center in Tehran

Sat Jun 25 03:12:40 2022

(2015) Low prevalence of metallo-beta-lactamase in Pseudomonas aeruginosa isolated from a tertiary burn care center in Tehran. International Journal of Immunopathology and Pharmacology. pp. 384-389. ISSN 0394-6320

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Abstract

Production of metallo-beta-lactamase (MBL) is one of the main mechanisms for resistance in carbapenem antibiotics. Detection of MBL-producer Pseudomonas aeruginosa is crucial in preventing its spread to other gram-negative bacteria. The aim of this study was to evaluate combination disc (CD) for identification of MBL-producer P. aeruginosa by polymerase chain reaction (PCR). A total of 255 imipenem resistant P. aeruginosa were collected from burn patients. Antibiotic susceptibility testing was conducted after purification and identification. Double-disc synergy test (DDST) with EDTA and combination disc test (CDT) with dipicolinic acid were performed for phenotypic detection of MBL and the PCR was carried out for bla(VIM), bla(IMP), bla(NDM-1), bla(SPM-1) genes. DDST with EDTA was negative in all cases, but 161 isolates were positive in CDT with dipicolinic acid. Further, bla(VIM) and bla(IMP) were detected in five and four strains, respectively. None of the isolates were positive for Bla(NDM-1) and bla(SPM-1). The results of this study showed that the prevalence of MBL is low in imipenem resistance P. aeruginosa and that other mechanisms could be involved in resistance to imipenem in this bacterium.

Item Type: Article
Creators:
CreatorsEmail
Lari, A. R.UNSPECIFIED
Azimi, L.UNSPECIFIED
Soroush, S.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Keywords: metallo-beta-lactamases phenotypic detection Pseudomonas aeruginosa acinetobacter-baumannii dipicolinic acid genes tests resistance bacteria oprd Immunology Pathology Pharmacology & Pharmacy
Divisions:
Page Range: pp. 384-389
Journal or Publication Title: International Journal of Immunopathology and Pharmacology
Journal Index: ISI
Volume: 28
Number: 3
Identification Number: https://doi.org/10.1177/0394632015578343
ISSN: 0394-6320
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/551

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