Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Prevalence of AmpC type extended spectrum beta lactamases genes in clinical Samples of E. coli Isolated from Poultry and Humans

(2016) Prevalence of AmpC type extended spectrum beta lactamases genes in clinical Samples of E. coli Isolated from Poultry and Humans. International Journal of Medical Research & Health Sciences. pp. 83-93. ISSN 2319-5886

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Emergence of antibiotic resistance among pathogens, particularly in health centers and hospitals, has become a major public health problem. This study identified AmpC-type beta-lactamase against the antibiotic ceftazidime, cefotaxime and cefpodoxime in E. coli isolated from human and poultry and types of producing genes were studied by PCR. In this study, 500 clinical human samples of urine from hospitals of Tehran during 5 months as well as 300 poultry samples were collected and transferred to the microbiology laboratory. Biochemical tests such as TSI, Urea and IMViC were performed on suspected colonies with E. coli. To identify ESBL producing strains, beta-lactamase samples were cultured on Mueller-Hinton agar through antimicrobial susceptibility test by disk agar diffusion based on the standard CLSI for initial screening. PCR reactions were done using specific primers CITM, EBCM, DHAM and MOXM to identify the beta-lactamase AmpC. A number of 200 human and 55 poultry E. coli samples were screened. In human samples, 105 (52.5) were resistant and potential producers of ESBL and AmpC; out of those, 102 (51) produced ESBL and 3 (1.5) potentially produced AmpC. In the study on 55 E. coli isolates from poultry samples based on the results of disk agar diffusion test, 4 (7.2) samples were resistant and potential producers of ESBL. None of the samples were AmpC producers. Through PCR, 2 human samples (1) were CITM positive and one sample (0.5) was DHAM positive. Through the PCR carried out on poultry samples, there were no bands with 4 primers. There was AmpC in human samples; while further studies are required for poultry samples, because poultry significantly contribute in production of food for humans and can be an important source for dissemination of antibiotic resistance. Given the significance of Ampc in providing high levels of beta-lactam antibiotic resistance, particularly third generation cephalosporins which are very common treatments, more extensive research is recommended.

Item Type: Article
Keywords: AmpC E. coli spectrum beta Poultry Humans resistant escherichia-coli molecular characterization susceptibility epidemiology hospitals infection Research & Experimental Medicine
Page Range: pp. 83-93
Journal or Publication Title: International Journal of Medical Research & Health Sciences
Journal Index: ISI
Volume: 5
Number: 7
ISSN: 2319-5886
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/515

Actions (login required)

View Item View Item