Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose-Induced Oxidative Stress

Wed Feb 28 13:47:54 2024

(2016) Biphasic Response to Luteolin in MG-63 Osteoblast-Like Cells under High Glucose-Induced Oxidative Stress. Iranian Journal of Medical Sciences. pp. 118-125. ISSN 0253-0716

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Background: Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether a cytoprotective concentration range of luteolin could be separated from a cytotoxic concentration range in human MG-63 osteoblast-like cells in high-glucose condition. Methods: Cells were cultured in a normal-or high-glucose medium. Cell viability was determined with the MTT assay. The formation of intracellular reactive oxygen species (ROS) was measured using probe 2',7'-dichlorofluorescein diacetate, and osteogenic differentiation was evaluated with an alkaline phosphatase bioassay. Results: ROS generation, reduction in alkaline phosphatase activity, and cell death induced by high glucose were inhibited by lower concentrations of luteolin (EC50, 1.29 +/- 0.23 mu M). Oxidative stress mediated by high glucose was also overcome by N-acetyl-L-cysteine. At high concentrations, luteolin caused osteoblast cell death in normal-and high-glucose states (IC50, 34 +/- 2.33 and 27 +/- 2.42 mu M, respectively), as represented by increased ROS and decreased alkaline phosphatase activity. Conclusion: Our results indicated that the cytoprotective action of luteolin in glucotoxic condition was manifested in much lower concentrations, by a factor of approximately 26 and 20, than was its cytotoxic activity, which occurred under normal or glucotoxic condition, respectively.

Item Type: Article
Keywords: Luteolin Human osteoblast-like MG-63 cells High glucose Oxidative stress Alkaline phosphatase mc3t3-e1 cells in-vitro diabetes-mellitus bone quercetin extract differentiation damage mineralization dysfunction General & Internal Medicine
Page Range: pp. 118-125
Journal or Publication Title: Iranian Journal of Medical Sciences
Journal Index: ISI
Volume: 41
Number: 2
ISSN: 0253-0716
Depositing User: مهندس مهدی شریفی

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