Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Investigation of biofilm formation ability, antimicrobial resistance and the staphylococcal cassette chromosome mec patterns of methicillin resistant Staphylococcus epidermidis with different sequence types isolated from children

Sun Sep 25 12:07:29 2022

(2016) Investigation of biofilm formation ability, antimicrobial resistance and the staphylococcal cassette chromosome mec patterns of methicillin resistant Staphylococcus epidermidis with different sequence types isolated from children. Microbial Pathogenesis. pp. 126-130. ISSN 0882-4010

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Abstract

This study investigated the molecular characterizations of 80 methicillin resistant Staphylococcus epidermidis (MRSE) collected during 2012-2013 in Tehran Children's Medical Center, Iran. About 90 of MRSE isolates were multi-drug resistant (MDR) and the highest resistance was observed to cotrimoxazole and they were quite sensitive to quinupristin-dalfopristin and linezolid. Though vanA gene was not detected, the majority of isolates showed intermediate resistance to vancomycin (MIC90 16 mu g/ml). Resistance to mupirocin was observed in 18 isolates. Staphylococcal cassette chromosome mec (SCCmec) types V, III, IV and II were detected in 23.75, 7.5, 6.25 and 5 of isolates respectively, in some of which the additional parts of mec or ccr complexes were observed. In 57.5 MRSE isolates SCCmec types were not classified. 41.2 of MRSE isolates were carrying intercellular adhesion (ica) operon and 40 had strong or intermediate biofilm. The types of arginine catabolic mobile element (ACME) were limited to type I and II. Nine sequence types (STs) were seen in mupirocin resistant MRSE isolates. The common STs were ST2, ST5 and ST22 with 27.7 (5/18), 22.2 (4/18) and 16.6 (3/18) frequencies, respectively. ST23, ST54 and ST179 plus three novels STs 580, 581,588 were also observed. The majority of STs, 83.3 (15/18) belonged to clonal complex 2 (CC2). The spread of antibiotic resistance and virulence factors among MRSE species is an alarming sign in Children's Hospitals. The combination of these two issues leads to increase the chance of successfully establishing of common STs in hospital environments, and promotes the device-related infections and bacteremia. (C) 2016 Elsevier Ltd. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Soroush, S.UNSPECIFIED
Jabalameli, F.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Amirmozafari, N.UNSPECIFIED
Fooladi, A. A. I.UNSPECIFIED
Asadollahi, K.UNSPECIFIED
Beigverdi, R.UNSPECIFIED
Emaneini, M.UNSPECIFIED
Keywords: MRSE MDR Biofilm ica operon ACME MLST coagulase-negative staphylococci sccmec type-iii variable-number tandem repeats health-care aureus epidemiology infections virulence susceptibility Immunology Microbiology
Divisions:
Page Range: pp. 126-130
Journal or Publication Title: Microbial Pathogenesis
Journal Index: ISI
Volume: 93
Identification Number: https://doi.org/10.1016/j.micpath.2016.01.018
ISSN: 0882-4010
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/475

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