Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits

Sun Jun 23 08:16:23 2024

(2022) Bioassay-guided isolation of phenolic apoptosis inducer anticancer agents from Berberis vulgaris fruits. Journal of Medicinal Plants. pp. 35-46. ISSN 16840240 (ISSN)

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Background: Cancer development is a multi-stage process in which apoptosis plays an important role. Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Berberis vulgaris L. fruits are a rich source of natural phenolic compounds and have been evaluated for their apoptotic effects. Objective: this study was carried out to evaluate the apoptotic effect of B. vulgaris fruits in normal and cancerous cells by investigating p53, Bax, and Bcl-2 genes expression and identifying an active compound. Methods: B. vulgaris fruits were extracted with ethanol using maceration method. To yield an active compound, the extract was divided into fractions and subfractions that could be evaluated using chromatography techniques, and the purified compound structure was determined by nuclear magnetic resonance (NMR). The cytotoxic activity of the selected fraction against 3T3 and MCF-7 cell lines along with its effect on the mRNA apoptosis regulating genes like p53, Bax, and Bcl-2 were investigated subsequently. Results: Results showed that B. vulgaris extract increased the mRNA level of p53 and Bax and decreased Bcl-2 mRNA level in cancer cell lines. The structure of the purified compound was determined as the 3-caffeoylquinic acid (chlorogenic acid). Conclusion: B. vulgaris fruit has in vitro antineoplastic activity by stimulating cellular apoptosis via increasing the levels of p53 and Bax proteins and decreasing Bcl-2 and comprises chlorogenic acid as one of its phenolic phytochemicals. © 2022 Institute of Medicinal Plants. All rights reserved.

Item Type: Article
Hosseinzadeh, L.UNSPECIFIED
Shokoohinia, Y.UNSPECIFIED
Keywords: Apoptosis Berberis vulgaris Cancer Chlorogenic acid NMR antimitotic agent apoptosis regulatory protein Berberis vulgaris extract complementary DNA doxorubicin messenger RNA phenol derivative plant extract protein Bax protein bcl 2 protein p53 unclassified drug 3T3 cell line analysis antineoplastic activity antiproliferative activity Article bioassay cell viability chemical composition controlled study cytotoxicity DNA synthesis drug isolation drug structure high performance liquid chromatography human human cell maceration method MCF-7 cell line mRNA expression level nuclear magnetic resonance phytochemistry real time polymerase chain reaction solvent extraction thin layer chromatography
Page Range: pp. 35-46
Journal or Publication Title: Journal of Medicinal Plants
Journal Index: Scopus
Volume: 21
Number: 83
Identification Number:
ISSN: 16840240 (ISSN)
Depositing User: مهندس مهدی شریفی

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