Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Cytokine changes and embryo attachment in mouse endometrial cells following treated with peripheral blood mononuclear cells (PBMCs) expressing ectopic hCG, and hCG-activated PBMCs

Wed Jun 19 21:43:56 2024

(2023) Cytokine changes and embryo attachment in mouse endometrial cells following treated with peripheral blood mononuclear cells (PBMCs) expressing ectopic hCG, and hCG-activated PBMCs. Asian Pacific Journal of Reproduction. pp. 90-96. ISSN 23050500 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Objective: To compare the effect of human chorionic gonadotropin (hCG)-producing peripheral blood mononuclear cells (PBMCs) and PBMCs activated by hCG in vitro and expressions of related immune genes in mouse implantation. Methods: hCG-producing PBMCs (transfected PBMC) and PBMCs activated by hCG in vitro were introduced into isolated mouse endometrial cells, while cell cultures were divided into four groups: the control, PBMC, transfected, and activated PBMC groups. The expression of studied genes (IL-1β, IL-6, Lif, and Vegf) was evaluated and blastocyst attachment on the cocultured cells (isolated endometrial cells and PBMC cells) was monitored in all four groups. Results: Data showed that expression decreased in the PBMC group compared to the treated PBMC (transfected and activated PBMCs) and increased in transfected PBMC compared to the activated PBMC. Attachment and migration of blastocysts were dramatically enhanced in the transfected PBMC group compared to the activated PBMC group (P<0.05). Conclusions: Use of hCG-producing PBMCs (transfected PBMC) has more influence on endometrial receptivity. ©2023 Asian Pacific Journal of Reproduction Produced by Wolters Kluwer- Medknow.

Item Type: Article
Creators:
CreatorsEmail
Rezaee, D.UNSPECIFIED
Bandehpour, M.UNSPECIFIED
Kazemi, B.UNSPECIFIED
Bastaminejad, S.UNSPECIFIED
Najafi, S.UNSPECIFIED
Salehi, M.UNSPECIFIED
Keywords: Embryo attachment Expression gene hCG Immune cells Immune response In vitro Peripheral blood mononuclear cells chorionic gonadotropin cytokine interleukin 1beta interleukin 6 leukemia inhibitory factor vasculotropin animal cell animal tissue Article blastocyst cell activation cell migration coculture comparative study controlled study embryo endometrium cell female gene expression level genetic transfection immunocompetent cell in vitro study mouse nidation nonhuman peripheral blood mononuclear cell protein expression
Divisions:
Page Range: pp. 90-96
Journal or Publication Title: Asian Pacific Journal of Reproduction
Journal Index: Scopus
Volume: 12
Number: 2
Identification Number: https://doi.org/10.4103/2305-0500.372380
ISSN: 23050500 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4533

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