Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

HIF-1α gene/protein and oxidative stress in patients with colorectal cancer: A pilot study

Mon May 20 15:33:14 2024

(2023) HIF-1α gene/protein and oxidative stress in patients with colorectal cancer: A pilot study. Gene Reports. ISSN 24520144 (ISSN)

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Background: Several risk factors such as may enhance the development of colorectal cancer (CRC). These include hypoxia and oxidative stress. We investigated serum pro-oxidant antioxidant balance (PAB) and HIF-1α Gene/protein levels in CRC patients over 3 months. Method: Fifty newly diagnosed cases of CRC and 50 healthy individuals were recruited. Tumor sections and marginal healthy tissue, for use as control tissue, were obtained on the day of surgery and HIF-1α mRNA gene expression was determined using Real-Time PCR. Blood from each patient was collected to measure serum PAB using a colorimetric assay, and serum HIF-1α protein concentrations were measured using ELISA method in the patient group before, 24 h after surgery, at the time of discharge, one month, and three months after surgery. The control group samples were obtained on the day of a routine check-up. Results: The expression of the HIF-1α gene in the tumor tissue was 7.31 times greater than in healthy tissue margins (p < 0.001) and the level of expression was significantly higher in men than woman (p = 0.006). The mean serum of HIF-1α protein levels and PAB values in patients significantly decreased after surgery up until 3 months (p < 0.001 and p < 0.001; respectively), and were not significantly different between the two genders during follow up (p > 0.05). Conclusion: HIF-1α gene expression was significantly higher in tumor tissue than normal marginal tissue and serum HIF-1α protein and PAB levels in patients with CRC were higher preoperatively than the control subjects, and decreased over the 3 months of follow-up. © 2023

Item Type: Article
Creators:
CreatorsEmail
Akbar, S.UNSPECIFIED
Fazeli, M. S.UNSPECIFIED
Hemmati, R.UNSPECIFIED
Pirzadeh, M.UNSPECIFIED
Seyedan, S. M.UNSPECIFIED
Ferns, G. A.UNSPECIFIED
Pakzad, R.UNSPECIFIED
Yazdanian, Z.UNSPECIFIED
Mobarra, N.UNSPECIFIED
Keywords: Colorectal cancer Hypoxia Hypoxia inducible factor Metastasis Pro-oxidant-antioxidant balance beta actin complementary DNA hypoxia inducible factor 1alpha messenger RNA tosylchloramide sodium adult Article blood analysis cancer patient cancer tissue clinical article clinical evaluation cohort analysis colon tissue colorimetry concentration process controlled study enzyme linked immunosorbent assay female follow up gene expression gene expression level hospital discharge human male medical examination metabolic parameters middle aged oxidative stress pilot study pro oxidant antioxidant balance prospective study protein blood level protein expression protein expression level real time polymerase chain reaction serum treatment duration
Divisions:
Journal or Publication Title: Gene Reports
Journal Index: Scopus
Volume: 32
Identification Number: https://doi.org/10.1016/j.genrep.2023.101783
ISSN: 24520144 (ISSN)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4518

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