Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In Silico Characterization of an Important Metacyclogenesis Marker in Leishmania donovani, HASPB1, as a Potential Vaccine Candidate

Fri May 24 10:16:06 2024

(2023) In Silico Characterization of an Important Metacyclogenesis Marker in Leishmania donovani, HASPB1, as a Potential Vaccine Candidate. BioMed research international. p. 3763634. ISSN 2314-6141 (Electronic) 2314-6133 (Print)

Full text not available from this repository.

Official URL: https://www.ncbi.nlm.nih.gov/pubmed/37323936

Abstract

Visceral leishmaniasis is a life-threatening infectious disease worldwide. Extensive experiments have been done to introduce potential vaccine candidates to combat leishmaniasis. The present study was done to evaluate Leishmania donovani hydrophilic acylated surface protein B1 as a potential vaccine candidate using in silico methods. For this aim, server-based predictions were performed regarding physicochemical characteristics, solubility, antigenicity, allergenicity, signal peptide, transmembrane domain, and posttranslational modifications (PTMs). Also, secondary and tertiary structures were predicted using NetSurfP-3.0 and I-TASSER, respectively. The 3D model was further subjected to refinement and validation, and promising B-cell, cytotoxic T-lymphocyte (CTL; human, dog), and helper T-lymphocyte (HTL; human) epitopes were predicted. The protein had a molecular weight of 42.19 kDa, with high solubility (0.749), stability (instability index: 21.34), and hydrophilicity (GRAVY: -2.322). No signal peptide or transmembrane domain was predicted, and the most abundant PTMs were phosphorylation, O-glycosylation, and acetylation. Many coils and disordered regions existed in the secondary structure analysis, and the tertiary model had a good confidence score (-0.79). Next, the ProSA-web and PROCHECK tools showed adequate improvements in the refined model compared to the crude model. Only 4 shared B-cell epitopes among three web servers (ABCpred, BepiPred 2.0, and SVMTriP) were shown to be antigenic, nonallergenic, and with good water solubility. Also, five potent CTL epitopes in dogs and five in humans were predicted. Notably, two HTL epitopes were found to be potential IFN-gamma inducers. In conclusion, our results demonstrated several immunogenic epitopes in this protein, which could be directed towards multiepitope vaccine design.

Item Type: Article
Creators:
CreatorsEmail
Kordi, B.UNSPECIFIED
Basmenj, E. R.UNSPECIFIED
Majidiani, H.UNSPECIFIED
Basati, G.UNSPECIFIED
Sargazi, D.UNSPECIFIED
Nazari, N.UNSPECIFIED
Shams, M.UNSPECIFIED
Keywords: Humans Animals Dogs *Leishmania donovani Epitopes, T-Lymphocyte Epitopes, B-Lymphocyte *Vaccines Peptides/chemistry Computational Biology Molecular Docking Simulation Vaccines, Subunit
Divisions:
Page Range: p. 3763634
Journal or Publication Title: BioMed research international
Journal Index: Pubmed
Volume: 2023
Identification Number: https://doi.org/10.1155/2023/3763634
ISSN: 2314-6141 (Electronic) 2314-6133 (Print)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4509

Actions (login required)

View Item View Item