Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

In silico analysis of sporozoite surface antigen 1 of Theileria annulata (TaSPAG1) for multi-epitope vaccine design against theileriosis

Wed May 22 11:30:23 2024

(2023) In silico analysis of sporozoite surface antigen 1 of Theileria annulata (TaSPAG1) for multi-epitope vaccine design against theileriosis. In silico pharmacology. p. 16. ISSN 2193-9616 (Print) 2193-9616 (Electronic) 2193-9616 (Linking)

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Official URL: https://www.ncbi.nlm.nih.gov/pubmed/37484780

Abstract

Tropical theileriosis is a protozoan infection caused by Theileria annulata, which significantly affects cattle worldwide. This study was aimed to analyze the TaSPAG1 protein and design a novel multi-epitope vaccine candidate. Online tools were employed for the prediction of Physico-chemical properties, antigenicity, allergenicity, solubility, transmembrane domains and signal peptide, posttranslational modification (PTM) sites, secondary and tertiary structures as well as intrinsically disordered regions, followed by identification and screening of potential linear and conformational B-cell epitopes and those peptides having affinity to bind bovine major histocompatibility complex class I (MHC-I) molecules. Next, a multi-epitope vaccine construct was designed and analyzed. This 907-residue protein was hydrophilic (GRAVY: -0.399) and acidic (pI: 5.04) in nature, with high thermotolerance (aliphatic: 71.27). Also, 5 linear and 12 conformational B-cell epitopes along with 8 CTL epitopes were predicted for TaSPAG1. The 355-residue vaccine candidate had a MW of about 35 kDa and it was antigenic, non-allergenic, soluble and stable, which was successfully interacted with cattle MHC-I molecule and finally cloned into the pET28a(+) vector. Further wet studies are required to assess the vaccine efficacy in cattle. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40203-023-00153-5.

Item Type: Article
Creators:
CreatorsEmail
Azimi-Resketi, M.UNSPECIFIED
Akbari, M.UNSPECIFIED
Heydaryan, S.UNSPECIFIED
Eftekhari, A.UNSPECIFIED
Balali, J.UNSPECIFIED
Shams, M.UNSPECIFIED
Sargazi, D.UNSPECIFIED
Keywords: Bioinformatics Immunogenic epitopes Spag1 Theileria annulata
Divisions:
Page Range: p. 16
Journal or Publication Title: In silico pharmacology
Journal Index: Pubmed
Volume: 11
Number: 1
Identification Number: https://doi.org/10.1007/s40203-023-00153-5
ISSN: 2193-9616 (Print) 2193-9616 (Electronic) 2193-9616 (Linking)
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4505

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