Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Paper Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture

Sat Jun 15 04:44:26 2024

(2022) Paper Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture. Basic and Clinical Neuroscience. pp. 625-636. ISSN 2008-126X

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Abstract

Introduction: The induction of human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases, including Parkinson disease. This study aims to differentiate HUC-MSCs into dopaminergic neuron-like cells.Methods: Following the isolation and characterization of HUC-MSCs, they were transferred to Matrigel-coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron -like cells in 2-dimensional culture and on Matrigel was assessed by real-time polymerase chain reaction, immunocytochemistry, and high-performance liquid chromatography.Results: Our results showed that dopaminergic neuronal markers' transcript and protein levels were significantly increased on the Matrigel differentiated cells compared to 2D culture plates. Conclusion: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potential for the treatment of dopaminergic neuron-related diseases.

Item Type: Article
Creators:
CreatorsEmail
Moayeri, A.UNSPECIFIED
Alizadeh, R.UNSPECIFIED
Hamidabadi, H. G.UNSPECIFIED
Bojnordi, M. N.UNSPECIFIED
Niapour, A.UNSPECIFIED
Hedayatpour, A.UNSPECIFIED
Darvishi, M.UNSPECIFIED
Heidari, F.UNSPECIFIED
Soleimani, M.UNSPECIFIED
Elyasi, L.UNSPECIFIED
Keywords: Differentiation Dopaminergic neurons Human umbilical cord mesenchymal stem cells In vitro assembling peptide nanofiber in-vitro differentiation bone-marrow long motif rat model es cells matrigel expression transplantation neurogenesis Neurosciences & Neurology
Divisions:
Page Range: pp. 625-636
Journal or Publication Title: Basic and Clinical Neuroscience
Journal Index: ISI
Volume: 13
Number: 5
Identification Number: https://doi.org/10.32598/bcn.2021.973.3
ISSN: 2008-126X
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4475

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