Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Spreading of genes encoding enterotoxins, haemolysins, adhesin and biofilm among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated from burn patients

Thu Aug 18 18:39:22 2022

(2016) Spreading of genes encoding enterotoxins, haemolysins, adhesin and biofilm among methicillin resistant Staphylococcus aureus strains with staphylococcal cassette chromosome mec type IIIA isolated from burn patients. Microbial Pathogenesis. pp. 34-37. ISSN 0882-4010

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Abstract

The emergence of antibiotic -resistant Staphylococcus aureus in particular methicillin-resistant S. aureus (MRSA) is an important concern in burn medical centers either in Iran or worldwide. A total of 128 S. aureus isolates were collected from wound infection of burn patients during June 2013 to June 2014. Multiplex-polymerase chain reaction (MPCR) assay was performed for the characterization of the staphylococcal cassette chromosome mec (SCCmec). Genes encoding virulence factors and biofilm were targeted by PCR. Of 128 S. aureus isolates, 77 (60.1) isolates were MRSA. Fifty four (70.1) isolates were identified as SCCmec type IIIA. The most frequently detected toxin genes among MRSA isolates with SCCmec type IIIA were sea (64.1) and hla (51.8). The rate of coexistence of sea with hla and sea with hla and hlb was 37 and 12.9, respectively. The sec, eta, tst, pvl, hla and hlb genes were not detected in any of the MRSA isolates. The most prevalent genes encoding biofilm was eno, found in 61.1 of isolates, followed by fib and icaA found in 48.1 and 38.8 of the isolates, respectively. The rate of coexistence of fib + eno + icaA + icaD and fib + eno was 20.3 and 9.2, respectively. The ebps gene was not detected in any of the isolates. In conclusion, our study indicated that the sea, hla, fib and icaA were most frequent genes encoding virulence factors among MRSA with SCCmec type IIIA isolated from burn wound infection. Moreover, the results of this study shows that the rate of coexistence of genes encoding different virulence factor were high. (C) 2016 Elsevier Ltd. All rights reserved.

Item Type: Article
Creators:
CreatorsEmail
Motallebi, M.UNSPECIFIED
Jabalameli, F.UNSPECIFIED
Asadollahi, K.UNSPECIFIED
Taherikalani, M.UNSPECIFIED
Emaneini, M.UNSPECIFIED
Keywords: MRSA SCCmec type IIIA MSCRAMMs Toxin genes Burn virulence factors multiplex pcr infections toxin epidermidis prevalence patterns brazil shock Immunology Microbiology
Divisions:
Page Range: pp. 34-37
Journal or Publication Title: Microbial Pathogenesis
Journal Index: ISI
Volume: 97
Identification Number: https://doi.org/10.1016/j.micpath.2016.05.017
ISSN: 0882-4010
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/447

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