Repository of Research and Investigative Information

Repository of Research and Investigative Information

Ilam University of Medical Sciences

Effect of siRNA-mediated silencing of p53R2 gene on sensitivity of T-ALL cellsto Daunorubicin

Thu Jun 20 23:38:42 2024

(2023) Effect of siRNA-mediated silencing of p53R2 gene on sensitivity of T-ALL cellsto Daunorubicin. Gene. p. 10. ISSN 0378-1119

Full text not available from this repository.

Official URL: http://apps.webofknowledge.com/InboundService.do?F...

Abstract

Introduction: p53R2 is a p53-inducible protein that, as one of the subunits of ribonucleotide reductase, plays an important role in providing dNTPs for DNA repair. Although p53R2 is associated with cancer progression, its role in T-cell acute lymphoblastic leukemia (T-ALL) cells is unknown. Therefore, in this study, we evaluated the effect of p53R2 silencing on double-stranded DNA breaks, apoptosis and cell cycle of T-ALL cells treated with Daunorubicin. Methods: Transfection was performed using Polyethyleneimine (PEI). Gene expression was measured using real-time PCR and protein expression was evaluated using Western blotting. Cell metabolic activity and IC50 were calculated using MTT assay, formation of double-stranded DNA breaks was checked using immunohistochemistry for & gamma;H2AX, and cell cycle and apoptosis were evaluated using flow cytometry. Results: We found that p53 silencing synergistically inhibited the growth of T-ALL cells by Daunorubicin. p53R2 siRNA in combination with Daunorubicin but not alone increases the rate of DNA double-strand breaks in T-ALL cells. In addition, p53R2 siRNA significantly increased Daunorubicin-induced apoptosis. p53R2 siRNA also caused a non-significant increase in cells in G2 phase. Conclusion: The results of the present study showed that silencing of p53R2 using siRNA can significantly increase the antitumor effects of Daunorubicin on T-ALL cells. Therefore, p53R2 siRNA has the potential to be used as an adjuvant therapy in combination with Daunorubicin in T-ALL.

Item Type: Article
Creators:
CreatorsEmail
Ghalesardi, O. K.UNSPECIFIED
Zaker, F.UNSPECIFIED
Ghotaslou, A.UNSPECIFIED
Boustani, H.UNSPECIFIED
Rezvani, M. R.UNSPECIFIED
Kiani, J.UNSPECIFIED
Shahidi, M.UNSPECIFIED
Keywords: Daunorubicin SiRNA T-cell acute lymphoblastic leukemia Ribonucleotide reductase acute lymphoblastic-leukemia small interfering rna expression progression Genetics & Heredity
Divisions:
Page Range: p. 10
Journal or Publication Title: Gene
Journal Index: ISI
Volume: 880
Identification Number: https://doi.org/10.1016/j.gene.2023.147622
ISSN: 0378-1119
Depositing User: مهندس مهدی شریفی
URI: http://eprints.medilam.ac.ir/id/eprint/4462

Actions (login required)

View Item View Item